­
­
­
­

Inventi Impact - Pharm Analysis & Quality Assurance

Articles

  • Inventi:ppaqa/27903/19
    DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF DARIFENACIN HYDROBROMIDE IN LIPOSOMES
    Y Padmavathi, Latha K, N Raghavendra Babu*, Menda Renuka, P Ravi Kumar

    A simple and sensitive liquid chromatographic method was developed for the estimation of darifenacin hydrobromide in liposomes. Analysis was carried out on Enable 18H C18 column using a mobile phase consisting of phosphate buffer pH 3 and acetonitrile (60:40 %v/v). The mobile phase was pumped at a flow rate of 1 ml/min and UV-detection was carried out at 284 nm. Linearity was observed over concentration range of 5-30 µg/ml and the coefficient of determination was found to be 0.9999. The proposed method was validated as per ICH Q2 (R1) guidelines. The limit of detection and limit of quantitation were found to be 0.24 and 0.73 ng/ml. The method was found to be linear, sensitive, precise, accurate and robust. The method has shown consistent and good recoveries from liposomes (98.96±0.88%) and tablet formulation (100.43±0.24%). The developed method was successively used for the quantitative analysis of darifenacin hydrobromide in the prepared liposomes.

    How to Cite this Article
    Y Padmavathi, Latha K, N Raghavendra Babu et al. Development and Validation of Liquid Chromatographic Method for Estimation of Darifenacin Hydrobromide in Liposomes. Inventi Impact: Pharm Analysis & Quality Assurance, 2019(2):54-60, 2019.
    Download Full Text