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Inventi Impact - Transplantation
(Formerly Inventi Rapid/Impact: Implants)

Articles

  • Inventi:hip/86/14
    THE MACROPHAGE INFLAMMATORY PROTEINS MIP1 (CCL3) AND MIP2 (CXCL2) IN IMPLANT-ASSOCIATED OSTEOMYELITIS: LINKING INFLAMMATION TO BONE DEGRADATION
    Ulrike Dapunt, Susanne Maurer, Thomas Giese, Matthias Martin Gaida, Gertrud Maria Hansch

    Bacterial infections of bones remain a serious complication of endoprosthetic surgery. These infections are difficult to treat, because many bacterial species form biofilms on implants, which are relatively resistant towards antibiotics. Bacterial biofilms elicit a progressive local inflammatory response, resulting in tissue damage and bone degradation. In the majority of patients, replacement of the prosthesis is required. To address the question of how the local inflammatory response is linked to bone degradation, tissue samples were taken during surgery and gene expression of the macrophage inflammatory proteins MIP1a (CCL3) and MIP2?? (CXCL2) was assessed by quantitative RT-PCR. MIPs were expressed predominantly at osteolytic sites, in close correlation with CD14 which was used as marker for monocytes/macrophages. Colocalisation of MIPs with monocytic cells could be confirmed by histology. In vitro experiments revealed that, aside from monocytic cells, also osteoblasts were capable of MIP production when stimulated with bacteria; moreover, CCL3 induced the differentiation of monocytes to osteoclasts. In conclusion, the multifunctional chemokines CCL3 and CXCL2 are produced locally in response to bacterial infection of bones. In addition to their well described chemokine activity, these cytokines can induce generation of bone resorbing osteoclasts, thus providing a link between bacterial infection and osteolysis.

    How to Cite this Article
    CC Compliant Citation: Ulrike Dapunt, Susanne Maurer, Thomas Giese, Matthias Martin Gaida, and Gertrud Maria Hänsch, “The Macrophage Inflammatory Proteins MIP1 (CCL3) and MIP2 (CXCL2) in Implant-Associated Osteomyelitis: Linking Inflammation to Bone Degradation,” Mediators of Inflammation, vol. 2014, Article ID 728619, 10 pages, 2014. doi:10.1155/2014/728619.
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