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Inventi Impact - OroDental

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  • Inventi:hod/80/14
    CANDIDA SPECIES DISTRIBUTION, GENOTYPING AND VIRULENCE FACTORS OF CANDIDA ALBICANS ISOLATED FROM THE ORAL CAVITY OF KIDNEY TRANSPLANT RECIPIENTS OF TWO GEOGRAPHIC REGIONS OF BRAZIL
    Walicyranison Plinio da Silva-Rocha, Vitor Luiz de Brito Lemos, Terezinha Ines Estivalet Svidizisnki, Eveline Pipolo Milan, Guilherme Maranhao Chaves

    Background: Candida albicans is a diploid yeast that in some circumstances may cause oral or oropharyngeal infections. This investigation aimed to study the prevalence of Candida spp. and to analyze the ABC genotypes of 76 clinical isolates of C. albicans obtained from the oral cavity of kidney transplant patients from two distinct geographic regions of Brazil. Methods: We typed 48 strains with ABC genotyping and Microsatelitte using primer M13 and tested three virulence factors in vitro: phospholipase activity, morphogenesis and the ability to evade from polymorphonuclear neutrophils phagocytosis. Results: C. albicans was the most prevalent species (86.4%), followed by C. tropicalis (4.5%). C. albicans genotype A was the most prevalent (58 isolates; 76.4%), followed by genotype C (15 isolates; 19.7%) and genotype B (3 isolates; 3.9%). When Microsatellite technique with primer M13 was applied, 80% of the isolates from the South were placed within the same cluster. The majority of Genotype C strains were grouped together within two different clusters. Genotype C was considered more resistant to PMNs attack than genotypes A and B. Strains isolated from the South of Brazil showed also better ability to combat PMNs phagocytosis. Conclusions: We found a high rate of C. albicans genotype C strains isolated from the oral cavity of this group of patients. This study characterized oral C. albicans strains isolated from kidney transplant recipients and will contribute to a better understanding of the pathogenesis of oral candidiasis.

    How to Cite this Article
    CC Compliant Citation: da Silva-Rocha et al.: Candida species distribution, genotyping and virulence factors of Candida albicans isolated from the oral cavity of kidney transplant recipients of two geographic regions of Brazil. BMC Oral Health 2014 14:20, doi:10.1186/1472-6831-14-20. © 2014 da Silva-Rocha et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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