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Inventi Impact - Planta Activa

Articles

  • Inventi:ppa/25058/18
    STUDY OF PHYTOCHEMICAL ANALYSIS, TOTAL FLAVONOID AND PHENOLIC CONTENT, ANTIMICROBIAL PROPERTIES AND CHEMICAL CONSTITUENTS OF TWO MANGLICOLOUS LICHENS EXTRACTS
    Vinay Bharadwaj Tatipamula*, Girija Sastry Vedula, Balaji B Rathod, Prakasham Reddy Shetty, A V S Sastry

    Graphis ajarekarii endemic crustose lichen and Parmotrema tinctorum foliose lichen, found growing lushly in India. Objective of this study was to establish the chemical and biological profile, total flavonoid and phenolic content of manglicolous lichens, G. ajarekarii and P. tinctorum. Initially, the hydroalcoholic extracts of manglicolous lichens, G. ajarekarii and P. tinctorum were prepared and re-extracted with n-hexane, chloroform, ethyl acetate and acetone. The resultant extracts were evaluated for their phytochemical analysis, total flavonoid (AlCl3 spectrophotometric method) and phenolic (Folin-Ciocalteu’s method) content, in-vitro antimicrobial (using four different bacterial and two fungal strains by cup plate method), antimycobacterial (by MABA) activities and the bioactive extracts were subjected to column chromatography. All the screened extracts showed differences in composition and pharmacological activities. Among all extracts, the bioactive profile, total flavonoid and phenolic content was noticed better with ethyl acetate extracts. Between ethyl acetate extract of G. ajarekarii showed prominence compared to P. tinctorum towards antimicrobial including against M. Tuberculosis H37Rv strain. The chemical isolation of ethyl acetate extracts of G. ajarekarii and P. tinctorum led to isolation of atranorin and ethyl everninate, respectively. This study reveals the relatively high levels of flavonoid, phenolic content and potent activity of G. ajarekarii against bacterial and fungal strains, while P. tinctorum was active against gram-negative strains. This data indicates that these manglicolous lichen might be a good source of antimicrobial and antimycobacterial agents.

    How to Cite this Article
    Vinay Bharadwaj Tatipamula, Girija Sastry Vedula, Balaji B Rathod et al. Study of Phytochemical Analysis, Total Flavonoid and Phenolic Content, Antimicrobial Properties and Chemical Constituents of Two Manglicolous Lichens Extracts. Inventi Impact: Planta Activa, 2018(3):129-134, 2018.
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