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Inventi Impact - Planta Activa

Articles

  • Inventi:ppa/28678/19
    PHYTOCHEMICAL INVESTIGATION AND COMPARATIVE ANTIMICROBIAL STUDIES ON DIFFERENT EXTRACTS OF BLACK GLUMED VARIETY NJAVARA (ORYZA SATIVA L.)
    Nagalekshmi R*

    Oryza sativa L. belongs to family poaceae. Black glumed variety of Njavara traditionally used effectively in the Ayurvedic system of medicine in certain specific treatments like Panchakarma. Scientific comparative studies on different extracts of Njavara rice and bran have not done. So we are interested to do phytochemical investigation and comparative antimicrobial studies on different extracts of black glumed variety of Njavara rice and bran. The different extracts of Njavara bran and rice were prepared using methanol, ethanol, chloroform and petroleum ether by maceration technique. The different extracts were subjected to preliminary phytochemical investigation. For the optimization purpose chromatographic technique like TLC were carried for different extracts. Comparative antimicrobial studies against E. coli, Bacillus subtilis and Klebsiella pneumoniae of different extracts were carried out using ofloxacin as standard by well diffusion method. Preliminary phytochemical investigations revealed the presence of alkaloids, saponins, flavanoids, Proteins and aminoacids, carbohydrates, tannins and phenolic compounds. From TLC using the mobile phase methanol: n-butanol: water in the ratio 45:1:2 methanolic, ethanolic, Pet. ether and chloroform extracts showed one, one, one and two spots respectively. From the comparative antimicrobial studies against E. coli ethanolic extracts showed maximum zone of inhibition than other extracts. Against Bacillus subtilis Chloroform extracts showed maximum zone of inhibition than other extracts. Against Klebsiella pneumoniae ethanolic extracts showed maximum zone of inhibition than other extracts.

    How to Cite this Article
    Nagalekshmi R. Phytochemical Investigation and Comparative Antimicrobial Studies on Different Extracts of Black Glumed Variety Njavara (Oryza sativa L.). Inventi Impact: Planta Activa, 2019(3): 131-133, 2019.
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