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Inventi Impact - Biomedical Analysis

Articles

  • Inventi:pba/15093/15
    HPLC METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF SITAGLIPTIN IN BLOOD PLASMA
    T Lakshmi*, A Annapurna, Krishna R Gupta

    A simple, precise and specific reverse phase high performance liquid chromatographic method has been developed and validated for the determination of sitagliptin in blood. The HPLC separation was carried out by reverse phase chromatography on Shimadzu HPLC system consisted of welchrom 5 ? C18 column (250 X 4.6 mm), SPD 10A UV detector and LC 10 AD pumps. Rheodyne injector with 20 ?l capacity, mobile phase comprises of methanol:water (65:35) pH 9.0 adjusted with triethanolamine at flow rate 1.0 ml/min. Glimperide was used as internal standard in the determination. The retention time of sitagliptin and internal standard was found to be 5.73 and 12.2 respectively. The detection was monitored at 262 nm. The calibration curve for sitagliptin was linear from 0.1-10 ?g/ml with correlation coefficient of 0.999. The intraday and interday precision was found to be within limits. The method was validated as per the guidelines.

    How to Cite this Article
    T Lakshmi, A Annapurna, Krishna R Gupta. HPLC Method Development and Validation for Determination of Sitagliptin in Blood Plasma. Inventi Impact: Biomedical Analysis, 2015(2):50-54, 2015.
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