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Inventi Impact - Biomedical Analysis

Articles

  • Inventi:pba/17841/15
    METHOD VALIDATION AND DETERMINATION OF EPRINOMECTIN IN SHEEP PLASMA AND MILK BY HPLC WITH FLUORESCENCE DETECTION
    C M Modi*, S K Mody, H B Patel, R D Singh

    The objective of the present study was to study the method validation and determination of eprinomectin in sheep plasma and milk following subcutaneous administration at a dose of 0.2 mg.kg-1. The study was conducted using six healthy lactating sheep. Eprinomectin is a novel and potent antiparasitic animal health drug. This paper describes a method for the determination of eprinomectin in sheep plasma and sheep milk by high-performance liquid chromatography (HPLC) with fluorescence detection. Extraction procedure involved treatment of samples with acetonitrile, than supernatant was cleaned up by Suplco SPE cartridge and finally dried elute was derivatized with N-methylimidazole, trifluoroacetic anhydride and acetic acid. Extracted samples were analyzed by HPLC with fluorescence detection of the excitation and emission wavelengths of 355 and 465 nm, respectively. After single dose subcutaneous administration eprinomectin, inter assay and intra assay mean accuracy of the method were 95.56% and 93.79% for plasma and 89.14% and 87.37% for milk sample, respectively. Where, inter assay and intra assay of precision C. V. was ranging from 0.25% to 3.14% and 1.27% to 8.01% for plasma and 1.11% to 5.46% and 3.55% to 6.46% for sheep milk.

    How to Cite this Article
    C M Modi, S K Mody, H B Patel et al. Method Validation and Determination of Eprinomectin in Sheep Plasma and Milk by HPLC with Fluorescence Detection. Inventi Impact: Biomedical Analysis, 2016(1): 6-10, 2016.
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