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Inventi Impact - Bioinformatics

Articles

  • Inventi:ebi/8836/14
    PREDICTION OF B-CELL EPITOPES IN LISTERIOLYSIN O, A CHOLESTEROL DEPENDENT CYTOLYSIN SECRETED BY LISTERIA MONOCYTOGENES
    Morris S Jones, J Mark Carter

    Listeria monocytogenes is a gram-positive, foodborne bacterium responsible for disease in humans and animals. Listeriolysin O (LLO) is a required virulence factor for the pathogenic effects of L. monocytogenes. Bioinformatics revealed conserved putative epitopes of LLO that could be used to develop monoclonal antibodies against LLO. Continuous and discontinuous epitopes were located by using four different B-cell prediction algorithms. Three-dimensional molecularmodels were generated to more precisely characterize the predicted antigenicity of LLO.Domain 4 was predicted to contain five of eleven continuous epitopes.Alarge portion of domain 4 was also predicted to comprise discontinuous immunogenic epitopes. Domain 4 of LLO may serve as an immunogen for eliciting monoclonal antibodies that can be used to study the pathogenesis of L. monocytogenes as well as develop an inexpensive assay.

    How to Cite this Article
    CC Compliant Citation: Morris S. Jones and J. Mark Carter, “Prediction of B-Cell Epitopes in Listeriolysin O, a Cholesterol Dependent Cytolysin Secreted by Listeria monocytogenes,” Advances in Bioinformatics, vol. 2014, Article ID 871676, 9 pages, 2014. doi:10.1155/2014/871676. Copyright © 2014 Morris S. Jones and J. Mark Carter. This is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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