­
­
­
­

Inventi Rapid - Pharm Analysis & Quality Assurance

Patent Watch

  • Fingerprint analysis using mass spectrometry

    The present invention is directed to a method for determining the presence of a residue on or within a fingerprint using matrix-assisted mass spectrometric techniques. The matrix-assisted mass spectrometric technique can be selected from Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) and/or Surface Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (SALDI-TOF-MS).

  • Method for graphically depicting drug adverse effect risks

    An enhanced method for displaying assessment and analysis of risks of adverse effects resulting from use of at least one substance of interest, comprising: identifying the at least one substance of interest; selecting a profile related to the safety of the at least one substance of interest, using at least one filter; analyzing the risks of adverse effects resulting from the use of the at least one substance of interest using at least one data mining engine; and displaying the results of the analysis of risks of adverse effects in a format that permits perception of correlations. Such a format is preferably at least one format selected from the group consisting of a radar display for display of correlations, a sortable table, and a sortable line listing and where the format contains elements linked to data regarding the adverse effects.

  • Multi-stage data processor with signal repeater

    A signal processing device having a plurality of processing stages, each of the plurality of processing stages being adapted for applying an input signal to each of at least one item under examination to be coupled to a respective one of the plurality of processing stages, and at least one signal reconditioning unit, each of the at least one signal reconditioning unit being adapted for reconditioning the input signal in a signal path between a preceding one of the plurality of processing stages and a subsequent one of the plurality of processing stages.

  • Method for determining a response of each probe zone on a test strip

    A method for determining a response of each probe zone on a test strip is provided. The present invention selects an average pixel value of each section of reference white respectively adjacent to the image of a target line to serve as a reference for determining a color response of the target line. When the color response is not less than a predetermined value, representing the target line has a positive response in response to a specific component of a tested solution tested by the test strip, and the specific component is present in the tested solution. The content of the specific component is proportional to the color response. When the color response is less than a predetermined value, representing the target line has a negative response in response to the specific component of the tested solution, and the specific component is absent in the tested solution.

  • Health management system and health management method

    A health care system includes a sampling device for sampling body fluid of a test subject, an analysis device for analyzing the sampled body fluid, and a transmission device for transmitting analytical data obtained from the analysis to a diagnosis unit, where the diagnosis unit is for diagnosing a health status of the test subject from the transmitted analytical data. Moreover, the system includes a server for storing and accumulating analytical data and/or diagnostic results, and a health-care-information display device for receiving and displaying diagnostic results.

  • Scalable integrated tool for compliance testing

    Methods, tools, systems and computer readable media for compliance testing instrumentation and/or software. Data from one or more analytical instruments and/or software is inputted, and calculations are performed on the data to produce one or more outputs. At least one of the outputs may be compared to first and second test limits, and compliance status of the at least one output relative to the first and second test limits is reported.

  • Tolerogizing compositions comprising botulinum toxin type B peptides

    The present invention provides BoNT/B peptides, BoNT/B peptide compositions, tolerogizing compositions, immune response inducing compositions, as well as methods of determining immunoresistance to botulinum toxin therapy in an individual, methods of treating immunoresistance to botulinum toxin therapy in an individual, methods of reducing anti-botulinum toxin antibodies in an individual and methods of inducing a BoNT/B immune response an individual.

  • Automated drug discrimination during dispensing

    The automated drug discrimination system inspects the drug being dispensed during the dispensing process so that the pharmacist can be certain the correct formulation, dosage and quality of pharmaceuticals were dispensed so the pharmacist does not need to spend as much time examining the dispensed drug. The pills are dispensed through a dispensing area using a dispensing apparatus and are collected in a collection area. At least two sensors take a plurality of measurements of an aggregate of the pills during the dispensing process or of each pill as it moves through the dispensing area. A discrimination system compares the measurements taken to verify that the pills dispensed are the type of pharmaceuticals intended to be dispensed as identified in the individual prescription for at least one of formulation and dosage of the pill.

  • Electrical connector apparatus and methods

    Size-specific electrical connector apparatus optimized for e.g., biomedical applications, and which prevents mating to the wrong sized terminals. In one embodiment, the connector apparatus comprises a device having a two piece assembly adapted to pivotally engage and lock a sensor such as those used in impedance cardiography (ICG). The conductor is shaped so as to provide a high degree of specificity in sensor connection as well as signal stability and reliability. In one variant, the pivot comprises a spring-loaded pivot having no external stops and the shape of a sensor engaging aperture is defined by a metal insert associated with one element of the assembly and the pivot is permanently formed within the connector. Thus, a user is obstructed from changing the properties of the connector to accept smaller or larger sensors. Methods of manufacturing and operating the connector are also disclosed. In one embodiment, an opening in the metal insert (212) is sized to exclude oversized sensors. In another embodiment, a V-shaped stop (308) on one of the elements (304) acts to limit closure and to prevent a tight grip upon an undersized sensor.

  • Quantitative measurement of isotope ratios by time-of-flight mass spectrometry

    A mass spectrometer includes a pulsed ion source that generates an ion beam comprising a plurality of ions. A first timed ion selector passes a first group of ions. A first ion mirror generates a reflected ion beam comprising the first group of ions that at least partially compensates for an initial kinetic energy distribution of the first group of ions. A second timed ion selector passes a second group of ions. A second ion mirror generates a reflected ion beam comprising the second group of ions that at least partially compensates for an initial kinetic energy distribution of the second group of ions. A timed ion deflector deflects the second group of ions to a detector assembly comprising at least two ion detectors which detects the deflected ion beam.

  • Printing fluid supply device with channeled absorbent material

    A printing fluid supply device for a printhead comprises a printing fluid storage compartment for storing printing fluid; an absorbent material provided in the printing fluid reservoir, the absorbent material comprising a plurality of channels formed therethrough; and a fluid trapping layer provided between the absorbent material and the printhead. The fluid trapping layer is adapted to absorb printing fluid and draw printing fluid from the printing fluid storage compartment into and through the absorbent material.

  • Radiation therapy plan dose perturbation system and method

    A method of determining a patient dose during or prior to therapy from an external radiation beam includes determining a dose distribution from a patient plan as delivered in a QA phantom at each appropriate beam angle and comparing the dose distribution determined from measurements or calculations to a corresponding treatment planning system (TPS) dose modeled distribution in the QA phantom and providing a correction distribution when applied to the TPS dose modeled distribution results in the dose distribution determined. The correction distribution may optionally be interpolated to non-measured points for each beam angle and geometrically projected toward the source of radiation through a volume that equals a dose volume of the TPS for a patient beam for each beam angle. The correction distribution is applied to the TPS patient dose volume for each beam angle for providing a corrected dose distribution in the patient.

  • Methods for detecting vitamin C by mass spectrometry

    Provided are methods for determining the amount of vitamin C in a sample using mass spectrometry. The methods generally involve ionizing vitamin C in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin C in the sample.

  • Fingerprint analysis using mass spectrometry

    The present invention is directed to a method for determining the presence of a residue on or within a fingerprint using matrix-assisted mass spectrometric techniques. The matrix-assisted mass spectrometric technique can be selected from Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) and/or Surface Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (SALDI-TOF-MS).

  • SUPERVISED PRINCIPAL COMPONENT ANALYSIS

    The invention provides a multivariate modeling method for quantitative analysis by supervised principal component analysis (SPCA). The method comprises: (a) designing a plurality of calibration samples wherein the desired variances are dominant or greatly enhanced; (b) producing a calibration data matrix using suitable mathematical pretreatment and truncation of the acquired NIR/Raman spectra of the calibration samples; (c) decomposing the matrix using PCA; (d) evaluating the score and loading matrices to ensure a genuine orthogonal relationship between scores of the desired latent variables in a two-dimensional principal component space 7; (e) generating a prediction matrix for quantitative prediction of unknown samples. This method does not require testing of calibration samples using a reference method. In addition, this method has high tolerance to variations in sample composition and manufacturing conditions.

  • PTEROSTILBENE COCRYSTALS

    Cocrystals of pterostilbene are disclosed, including: pterostilbene:caffeine cocrystal, pterostilbene:carbamazepine cocrystal, pterostilbene:glutaric acid cocrystal, and pterostilbene:piperazine cocrystal. The pterostilbene:caffeine cocrystal is polymorphic. Forms I and II of the pterostilbene:caffeine cocrystal are disclosed. The therapeutic uses of the pterostilbene cocrystals and of pharmaceutical/nutraceutical compositions containing them are also disclosed. The disclosure sets out various methods of making and characterizing the pterostilbene cocrystals.

  • ENHANCING SIGNALS

    A method of testing a sample comprising the steps of: applying an excitation to the sample; detecting a response signal from the sample; processing a first part and a second part of the response signal; and determining from the second part of the response signal information with which to enhance the first part of the response signal.

  • Simulation Distillation by Comprehensive Two-Dimensional Gas Chromatography

    A method to simulate distillation of a petroleum stream by comprehensive two-dimensional gas chromatography including the step of separating said petroleum stream with a two-dimensional gas chromatograph to determine polarity as a function of temperature, and integrating vertically the two-dimensional gas chromatograph at a given temperature to determine signal intensity as a function of temperature.

  • METHOD FOR ANALYSING A COMPLEX SAMPLE BY MASS SPECTROMETRY

    The present invention pertains to a mass spectrometry method for analysing the presence or absence of at least one target analyte in a complex sample, comprising at least the following steps: a) Using a sample carrier suitable for mass spectrometry comprising a pre-applied microcrystalline MALDI matrix spot which is at least partially encompassed by a hydrophobic region; b) Applying a complex sample such that it becomes located on said microcrystalline MALDI matrix spot; c) washing said sample on said microcrystalline MALDI matrix spot; d) analysing said sample for the presence or absence of at least one target analyte via mass spectrometry. The method is in particular suitable for quantitatively analysing target analytes such as hepcidin and other peptides, drug compounds and metabolites in body fluids.

  • SPECTRAL IMAGING OF PHOTOLUMINESCENT MATERIALS

    A near infrared imaging and detection system is configured to analyze shifts in photoluminescence of individual nanostructures such as single-walled carbon nanotubes or quantum dots upon binding an analyte. The system can be used to detect, localize, and quantify analytes down to the single-molecule level in a sample and within living cells and can be operated in a multiplex format. The system also can be configured to perform high-throughput chemical analysis of a large number of samples simultaneously. The invention has application in the highly sensitive diagnosis of disease, as well as the detection and quantitative analysis of drugs, molecular pathogens within a living organism, and environmental toxins.

  • NMR METHOD FOR DIFFERENTIATING COMPLEX MIXTURES

    A method for differentiating complex mixtures each having one or more chemical species is provided. The method comprises producing a sample NMR spectrum by subjecting a mixture to a selective spectroscopy process, wherein the NMR spectrum has individual spectral peaks representative of the one or more chemical species within the mixture. The one or more chemical species within the mixture are identified by analyzing the individual spectral peaks, and the individual spectral peaks are then subjected to a multivariate statistical analysis.

  • Server for Integrated Pharmaceutical Analysis and Report Generation Service, Method of Integrated Pharmaceutical Manufacturing and Research and Development Numerical Analysis, and Computer Readable Recording Medium

    A web-based tool (as a server) for integrated pharmaceutical analysis and report generation service is provided in the present invention. The server can be used for numerical analysis and report generation for pharmaceutical manufacturing, research and development, and has advantages such as simple operation, complicated but fast calculation and professional report generation, and high accuracy. The server includes at least one pharmaceutical manufacturing and research and development numerical analysis system configured to perform different pharmaceutical manufacturing and research and development numerical analyses and generate different reports. Each of the at least one pharmaceutical manufacturing and research and development numerical analysis system includes an input module configured to receive, via a user interface, at least one of a template file and a backup file previously output by the server as at least one input file, wherein the at least one input file includes a plurality of data fields to provide corresponding data; at least one calculation module, each configured to execute a built-in pharmaceutical manufacturing and research and development numerical analysis calculation program, thereby automatically performing a pharmaceutical manufacturing and research and development numerical analysis calculation on at least one of the data of the at least one input file and on-line filled data; and an output module configured to generate at least one of a backup file and a report file as at least one output file based on the result of the pharmaceutical manufacturing and research and development numerical analysis calculation performed by the at least one calculation module and provide the at least one file via the user interface.

  • Method of Detecting Filter Extractables in Biopharmaceutical Products By Liquid Chromatography-Mass Spectrometry

    The invention uses liquid chromatography-mass spectrometry to verify the presence or absence of the extractables introduced into a biopharmaceutical product during manufacture, filtration or storage at above a certain concentration. The method disclosed herein can be used to detect the presence of extractables in the actual biopharmaceutical product rather than a surrogate solvent system. Detection of the extractables in the actual drug product provides an accurate description of the leachables profile likely to be introduced to the human or animal patient. In one embodiment, the extractable are introduced by filtration of the bio-pharmaceutical product.

  • ANALYSIS OF MYCOPHENOLIC ACID IN SALIVA USING LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY

    A method for mass spectrometric analysis of a saliva sample possibly containing mycophenolic acid or its metabolites mycophenolic acid phenyl glucuronide (MPAG) or mycophenolic acid acyl-glucuronide (Acyl-MPAG), including the steps: (a) providing a saliva sample containing one or more drug or metabolites; (b) deproteinating the sample; (c) separating the one or more drug or metabolites from the saliva sample; and (d) analyzing the one or more drug or metabolites using a mass spectrometer. The sample containing one or more MPA or metabolites is obtained from in an oral fluid based biological samples i.e. whole saliva or saliva obtained by chemical or mechanical stimulation or from specific salivary glands. The size of the sample contains one or more MPA or metabolites is at least about 100 microL. A kit for use in mass spectrometric analysis of a sample may contain one or more MPA or metabolites from saliva samples, comprising: (a) reagents for deproteinating of the saliva sample, including internal standards; (b) reagents for separating the one or more MPA or metabolites from the saliva sample; (c) reagents for analyzing the one or MPA or metabolites using a mass spectrometer; (d) a solution of one or more MPA or metabolites in saliva samples; and (e) instructions for analyzing the one or more MPA or saliva using a mass spectrometer. The kit includes (a) mobile phase solutions; (b) a chromatography column; and (c) a quality control specimen.

  • Spectrometer, Measuring Apparatus, and Method of Data Processing

    A spectrometer has: Accumulation means to obtain a data set containing N data points, repeating the measurement M times to obtain M spectral data sets or time-domain data sets S1 (d1 to dN) to SM (d1 to dN), and accumulating the M spectral data sets or time-domain data sets. Means for creating sets S1 (dn) to SM (dn) of the data points contained in the M spectral data sets or time-domain data sets S1 (d1 to dN) to SM (d1 to dN). Correlation computing means for finding correlations. Computing means for finding either the product of an accumulated or anticipated spectrum.

  • PREDICTION OF WAX APPEARANCE TEMPERATURE AND SOLID WAX AMOUNT BY REDUCED SPECTRAL ANALYSIS USING FTIR SPECTROSCOPY

    A method that uses corrected areas integrated at two different wavelength ranges, 1402-1324 cm.sup.-1 and 735-715 cm.sup.-1. The invention uses the reduced form of FTIR spectral integration. The invention provides reliable data in the variety of applications regardless of FTIR spectral instability occurring unexpectedly, such as loading sample thickness, sample cell location changes of FTIR light source passes, volume changes during cooling procedure, existence of emulsified water, moisture building on the surface of FTIR crystals.

  • IMMUNOANALYTICAL METHOD AND SYSTEM USING MASS SPECTROMETRY TECHNOLOGY

    An immunoanalytical system in which, after a sample such as a patient's serum is subjected to a pretreatment by an immunological pretreatment device, the sample is subjected to light detection by an immunological photometric detection system. Subsequently, the mass spectrometric pretreatment device performs a pretreatment, and the mass spectrometric detection system performs mass spectrometry. The mass spectrometric detection system performs mass spectrometry on components contained in a supernatant. A signal intensity and peak area for each of components are calculated from an obtained chromatograph. A quantitative value measured based on the immunoanalytical method is calculated for each of the components on the basis of the relative ratios of the components.

  • QUANTIFICATION OF ENZYME ACTIVITY BY MASS SPECTROMETRY USING IMMOBILIZED SUBSTRATES

    The disclosure relates to methods of analyzing the enzymatic activity of enzymes in samples containing a plurality of enzymes, using mass spectrometry. Immobilized substrates are employed. Purified enzymes and enzymes from crude cell lysates can be analyzed using the disclosed methods.

  • Determination of Tissue States by Imaging Mass Spectrometry

    Spatially resolved tissue states (status image) are determined from spectrally resolved mass spectra of a tissue section by (a) acquiring a plurality of spatially resolved mass spectra of the tissue section, (b) generating at least two mass images from the spatially resolved mass spectra, (c) smoothing the mass images using an edge-preserving smoothing algorithm and (d) calculating a status image from the smoothed mass images by means of a classification algorithm derived from mathematical statistics.

  • SPECTROMETRY APPARATUS, DETECTION APPARATUS, AND METHOD FOR MANUFACTURING SPECTROMETRY APPARATUS

    A spectrometry apparatus includes a transmissive diffraction grating that transmits incident light. The transmissive diffraction grating has inclined surfaces made of a first dielectric material. The inclined surfaces are arranged so that they are inclined relative to a reference line. When the angle of incidence of light incident on the transmissive diffraction grating is measured with respect to the reference line and defined as an angle .alpha., and the angle of diffraction of diffracted light is measured with respect to the reference line and defined as an angle .beta., the angle of incidence .alpha. is smaller than a Bragg angle .theta. defined with respect to the inclined surfaces, and the angle of diffraction .beta. is greater than the Bragg angle .theta..

  • SYSTEM AND METHOD OF DATA-DEPENDENT ACQUISITION BY MASS SPECTROMETRY

    Systems, computer-readable media, and methods using mass spectrometry to analyze a sample are provided. For example, a method includes: acquiring a precursor ion spectrum; analyzing the precursor ion spectrum to identify precursor ions that preliminarily match one or more peptides that each belong to at least one protein of interest for the analysis; selecting each of the identified precursor ions in an order according to a ranking protocol for maximizing the number of proteins that are identified in the sample; for each selected precursor ion: acquiring a corresponding product ion spectrum, determining whether the acquired product ion spectrum matches one of the peptides that belong to the set of proteins of interest, and identifying a matched peptide as being present in the sample; and identifying proteins of interest that are present in the sample based on the peptides that are identified as being present in the sample.

  • SEPARATION COLUMN WITH GERMANIA-BASED SOL-GEL STATIONARY PHASE

    The present invention provides sol-gel germania-coated chromatography separation columns with improved stability under extreme pHs and high temperatures. Also provided are methods of preparation and uses of the sol-gel germania-coated separation columns of the present invention.

  • Chromatography Media And Method

    Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

  • ION CHROMATOGRAPHY SYSTEM USING CATALYTIC GAS ELIMINATION

    A liquid chromatographic system is provided including catalytically combining hydrogen and oxygen gases in the chromatography eluent stream in a catalytic gas elimination chamber, to form water and thereby reduce the gas content in the eluent stream. Also, a liquid ion chromatographic system in which the effluent from the detector is recycled to a membrane suppressor and then is mixed with a source of eluent for recycle to the chromatographic column.

  • IONIZABLE ISOTOPIC LABELING REAGENTS FOR RELATIVE QUANTIFICATION BY MASS SPECTROMETRY

    Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications. Labeled primary and secondary amine and carboxylic acid end-products generated higher signals concerning mass-spectra than pre-cursor molecules and improved sensitivity. Improved accuracy concerning relative quantification was demonstrated by mixing heavy and light labeled Arabidopsis extracts in different ratios.

  • SENSITIVE METHOD TO ANALYSE FREE PEG-MALEIMIDE

    The present invention relates generally to a novel method using HPLC and fluorescence detection of free PEG-mal in PEGylated proteins and PEG-mal raw materials by adding a fluorescent label to the free PEG-mal.

  • METHOD FOR GAS CHROMATGRAPHY ANALYSIS AND MAINTENANCE

    A method of analyzing gas chromatography data may include acquiring response factor data for each of a plurality of compounds included in one or more calibration gas samples from a gas chromatograph apparatus, and determining a correlation with molecular weight data for each of the plurality of compounds. The correlation may be analyzed to determine a condition of the gas chromatograph. The method may also determine a correlation for each of a plurality of operating phases of the gas chromatograph, for example, before and after actuation of valves which change the flow rate. The method may also include diagnosing faults, and calibrating and configuring gas chromatographs.

  • Diluent for Preparing Analytical Sample

    The ionic strength of a diluent for preparing an analytical sample is set to be 0.06 to 0.16. The analytical sample prepared by using the diluent having the ionic strength within this range can be subjected to both for analyzing a first object in a test sample by electrode method and for analyzing a second object in the test sample by liquid chromatography method, and high-precision measurement can be attained. The analytical sample is especially useful for preparing a sample for measurement used both for measuring glucose concentration in a blood sample by enzyme electrode method and for measuring glycohemoglobin concentration in the blood sample by liquid chromatography method.

  • METHOD FOR IMPROVING THE EFFICIENCY OF HIGH-PRESSURE LIQUID CHROMATOGRAPHY

    The present invention relates to a method for improving the efficiency of high-pressure liquid chromatography columns in HPLC. More specifically, the method of the present invention provides a high-pressure liquid chromatography column being divided into a multitude of shorter separation segments, the shorter separation segments being serially connected with cooling segments. By applying an active controlled cooling action on the fluid flow passing through the cooling segments the separation efficiency of the high-pressure liquid chromatography column can be increased significantly.

  • PROCESS FOR THE PURIFICATION OF AMOROLFINE HYDROCHLORIDE

    The present invention relates to a process for the purification of amorolfine hydrochloride by means of a reversed-phase preparative high performances liquid chromatography (prep-HPLC) said method starting from a crude Amorolfine hydrochloride having purity higher than 90% and containing Bepromoline hydrochloride <5% and Fenpropimorf <3%. The process involves the use of a mobile phase comprising water and an organic solvent under isocratic conditions.

  • Method for Determining Chromium Content in a Tungsten Matrix with Added Chromium or Simultaneously Added Chromium and Vanadium

    A method for determining chromium content in a tungsten matrix with singly or simultaneously added chromium and vanadium, characterized in that a test sample is subjected to melting with sodium peroxide and hot water leaching; meanwhile said sodium peroxide is also used as an oxidizing agent to oxidize all the chromium to high valences; the main body of tungsten is coordinated by ammonium hydrogen fluoride to prevent tungstic acid from precipitating; precipitation and turbidity are avoided throughout the analysis, titration can be carried out in a quite clear condition with accurate and reliable results. In this invention, the clearness of the solution when titrating is very important for the accuracy of titrimetric analysis; the solution is always clear throughout the determination, ensuring that the interference with determination of chromium from vanadium is accurately and quantitatively eliminated; the interference from vanadium is eliminated by subtraction method, by means of titrating with ferrous ammonium sulfate standard solution after oxidation with potassium permanganate. In this invention, the determination method allows the determination to be carried out in the quite clear condition, and eliminates the interference from vanadium completely and quantitatively, thus the accuracy and speed of the determination of chromium content in a tungsten matrix with singly or simultaneously added chromium and vanadium are improved.

  • Gentamicin Separation Method

    The invention provides more effective methods of separating the components of gentamicin using a UV active protecting group suitable for use with HPLC.

  • IDENTIFICATION OF INSECT ATTRACTANT, ARRESTING, AND/OR AGGREGATION COMPOUNDS AND METHODS THEREOF

    Compounds, compositions, kits, devices, and methods of attracting, detecting, eradicating, controlling, or killing an insect, such as a bed bug, by utilizing insect attractant, arresting, and/or aggregation compounds and compositions is provided. Insect attractant, arresting, and/or aggregation compounds identified from insect fecal extract by an analytical technique, such as gas chromatography, nuclear magnetic resonance (NMR), Carbon-13 NMR, mass spectroscopy, LC-MS, GC-MS, high performance liquid chromatography (HPLC), or combinations thereof are provided. A bed bug attractant, arresting, and/or aggregation compound identified from bed bug feces and exhibiting a Carbon-13 NMR peak at about .delta. 159.453 ppm is also provided.

  • SOLVENT DELIVERY PUMP AND LIQUID CHROMATOGRAPH

    A cleaning chamber is provided in a seal holder and two cleaning chamber flow paths communicating with the cleaning chamber from outside are provided. A pipe connected to a vessel storing a mobile phase is connected to one of the cleaning chamber flow paths. A pipe as a flow path connected to a mobile phase sucking flow path into a pump chamber is connected to the other cleaning chamber flow path. When the mobile phase is taken in, the mobile phase is sucked into the pump chamber via the cleaning chamber.

  • LIQUID CHROMATOGRAPH

    In injecting a sample sucked into a sample loop in an autosampler from an injection needle into an analytical flow path via an injection port by a mobile phase, a sending flow rate of the mobile phase is reduced from a certain flow rate determined in an analysis condition to reduce pressure applied on a joint section between the needle and the injection port to thereby reduce the sample remaining in the injection port.

  • METHODS FOR REMOVING A CONTAMINANT USING INDIGENOUS PROTEIN DISPLACEMENT ION EXCHANGE MEMBRANE CHROMATOGRAPHY

    Methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant are described, which methods comprise the sequential steps of: (a) passing the composition through an ion exchange membrane, where the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and the at least one contaminant, and (b) recovering the purified polypeptide from the effluent.

  • METHOD FOR THE PURIFICATION OF PROTEIN COMPLEXES

    The present invention provides an improved method for the purification of a mixture of complexes comprising a stress protein complexed to a peptide or peptide fragment from a source mixture, typically a cell lysate. The method of the invention provides for protein complexes to be purified using ion exchangechromatography based methods, wherein a modified buffer solution is used which results in the purified stress protein complexes being more immunogenic than protein complexes obtained using conventional methodology. The purified complexes can be used to produce improved vaccine preparations which elicit enhanced immune responses in the subjects to whom the vaccine compositions are administered.

  • MIXTURES OF AND METHODS OF USE FOR POLYUNSATURATED FATTY ACID-CONTAINING PHOSPHOLIPIDS AND ALKYL ETHER PHOSPHOLIPIDS SPECIES

    A chemical composition of a molecular species mixture of phospholipids, purified to at least 85% purity through chromatography purification, the chemical composition contains enriched both sn-1-acyl fatty chains/sn-2-docosahexaenoic acid molecular species and sn-1-ether fatty chains/sn-2-docosahexaenoic acid molecular species, the phospholipids are selected from the group consisting of: phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine. Methods using the above disclosed composition in mammals to treat various conditions.

  • X-Ray Characterization of Solid Small Molecule Organic Materials

    The present invention provides, inter alia, methods of characterizing a small molecule organic material, e.g., a drug or a drug product. This method includes subjecting the solid small molecule organic material to x-ray total scattering analysis at a short wavelength, collecting data generated thereby, and mathematically transforming the data to provide a refined set of data.

  • PHARMACEUTICAL DATA INTEGRATION & ANALYSIS TOOL

    A system and processes are used to strategically evaluate opportunities for developing and producing generic pharmaceuticals. The system includes a knowledge driven optimization tool that efficiently analyzes pharmaceutical opportunities after merging pharmaceutical records from multiple sources. The analytical system allows users to filter the integrated records and evaluate the integrated records using patterns in the data. The system calculates historical trends of generic pharmaceutical compositions, and a user can select a market entry template that is based on the historical trends which the system then applies to a current pharmaceutical composition to create a forecast.

  • PHARMACEUTICAL DATA INTEGRATION & ANALYSIS TOOL

    A system and processes are used to strategically evaluate opportunities for developing and producing generic pharmaceuticals. The system includes a knowledge driven optimization tool that efficiently analyzes pharmaceutical opportunities after merging pharmaceutical records from multiple sources. The analytical system allows users to filter the integrated records and evaluate the integrated records using patterns in the data. The system calculates historical trends of generic pharmaceutical compositions, and a user can select a market entry template that is based on the historical trends which the system then applies to a current pharmaceutical composition to create a forecast.

  • Techniques For Sample Analysis

    Techniques are described for performing sample analysis. Liquid chromatographic separation of a sample is performed and an eluent is generated. Mass spectrometry on said eluent is performed to detect a compound where the compound may occur in trace amounts. The compound may have a concentration, for example, of approximately less than one part per trillion.

  • SPECTRAL ANALYSIS OPERATING SYSTEM

    Collecting and analyzing spectral data can be challenging when multiple analysis instruments need to be integrated and monitored by a quality control agent within a laboratory, industrial plant, field operation, or even an aerospace environment. The spectral analysis system and method, as presented, provides improved quality control, process control, and data management through unique feedback mechanisms between all hardware and software components within an analytical environment. Through spectral analysis presented, meaningful information is extracted from a spectral signal and fed back into the spectral analysis system to enhance overall system performance. A centralized database is provided to allow multiple users the opportunity to query the database for historical spectral records that can lead to the generation of meaningful reports. Additional hardware can be adapted to the present spectral analysis system in order to monitor a variety of physical phenomena in addition to monitoring a portion of the electromagnetic spectrum.

  • SINGLE PARTICLE QCL-BASED MID-IR SPECTROSCOPY SYSTEM WITH ANALYSIS OF SCATTERING

    This disclosure concerns a system with scattering analysis including a handling system that presents a single particle to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light to the single particle in order to induce resonant mid-infrared absorption in the particle or an analyte within the particle. A mid-infrared detection facility detects the mid-infrared wavelength light scattered by the single particle, wherein a wavelength and angle analysis of the scattered mid-IR wavelength light is used to determine analyte-specific structural and concentration information.

  • SINGLE PARTICLE QCL-BASED MID-IR SPECTROSCOPY SYSTEM WITH ANALYSIS OF SCATTERING

    This disclosure concerns a system with scattering analysis including a handling system that presents a single particle to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light to the single particle in order to induce resonant mid-infrared absorption in the particle or an analyte within the particle. A mid-infrared detection facility detects the mid-infrared wavelength light scattered by the single particle, wherein a wavelength and angle analysis of the scattered mid-IR wavelength light is used to determine analyte-specific structural and concentration information.

  • ELECTROPHORETIC ANALYSIS METHOD

    Provided is a means for accurately analyzing a protease by electrophoresis. Disclosed is an electrophoretic analysis method for analyzing a protease-containing sample, is characterized by exposing a sample containing a protease to be analyzed, to pH conditions under which the protease is rapidly deactivated, and then subjecting the sample to electrophoresis.

  • Method for the qualitative and/or quantitative analysis of tumour cells

    A method for qualitative and/or quantitative analysis of tumor cells, including the following steps: a) generating a dye complex from an aminocoumarin and cyclodextrin; b) mixing the tumor cells with the dye complex prepared in step a); c) incubating the cells with the dye complex prepared in step a); d) analyzing the tumor cells by means of fluorescence microscopy and/or fluorescence spectrometric analysis.

  • Methods and systems for analysis of nutraceutical associated components

    The present disclosure relates to methods and systems that may be used for analysis of nutraceutical associated components.

  • Electron Transfer Dissociation for Biopolymer Sequence Analysis

    The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.

  • METHODS FOR DIAGNOSING CHRONIC DIARRHEA THROUGH PROTEIN SECRETION ANALYSIS

    Methods for diagnosing chronic diarrhea and other gastrointestinal conditions. In the methods, a sample of gastrointestinal secretions is obtained from a control group; or a group who has been diagnosed with either healthy gastrointestinal tracts or with a gastrointestinal condition, like chronic diarrhea. The control group samples are analyzed in any suitable manner to determine the levels of gastrointestinal secretions, including one or more autophagy-related proteins, cytokeratins, digestive enzymes, or other proteins. The results of the sample analysis are used to create a database containing profiles of normal and abnormal gastrointestinal secretions. As the database is created and specific secretion level abnormalities are identified, patients may be diagnosed with these abnormalities and be treated by adjusting the levels of specific secretions. Gastrointestinal samples from subsequent patients may be analyzed and compared with the database to determine which of the patients' secretion levels, if any, are abnormal.

  • SYSTEM AND METHOD FOR MACHINE BASED MEDICAL DIAGNOSTIC CODE IDENTIFICATION, ACCUMULATION, ANALYSIS AND AUTOMATIC CLAIM PROCESS ADJUDICATION

    A context sensitive methodology, a Structured Virtual Construct (SVC) system, data tagging techniques, and an apparatus are provided for performing Medical Code-based decision-making involving the matching of a given medical identified element against one or more of a set of known or reference medical identified elements from history or other data elements. A satisfactory decision is achieved as a function of both aggregated ranking (AR) and account adjudication (AA), where account adjudication refers to the full set of values garnered by the Medical Code accumulation process in the process of generating approval/denial/re-classification/of medical diagnosis and/or claim events.

  • OPTICAL INFORMATION ANALYZER AND OPTICAL INFORMATION ANALYSIS METHOD

    An optical information analyzer (10) includes a light emitting unit (30) that emits light (excitation light) (L0) to a sample (S), a transmission light receiving unit (50) that receives transmission light (L1), which is the excitation light passing through the sample (S), and detects the received transmission light as a transmission light signal (SG1), scattered light/fluorescence receiving units (60) and (70) that are provided at a plurality of positions, receive side scattered light/fluorescence components (L2) and (L3) from the sample (S), and detect the received side scattered light/fluorescence components as scattered light/fluorescence signals (SG2) and (SG3), and an analyzing unit (90) that measures the optical information of the sample (S) on the basis of the detected scattered light/fluorescence signals (SG2) and (SG3) and the detected transmission light signal (SG1) and analyzes the sample (S) on the basis of the measured optical information.

  • VASCULAR ANALYSIS METHODS AND APPARATUS

    According to some aspects, a method of identifying a boundary of a portion of a vasculature is provided, the vasculature comprising a geometric representation of a plurality of vessels. The method comprises logically dividing the geometric representation into a plurality of regions, determining at least one feature within each of the plurality of regions, and defining the boundary of the portion of the vasculature based, at least in part, on the at least one feature determined within each of the plurality of regions, wherein the boundary forms a volume defining a separation between inside and outside of the portion of the vasculature. According to some aspects, a method of performing vascular analysis using a geometric representation of a plurality of vessels of the vasculature is provided. The method comprises computing a boundary of a portion of the vasculature based on the geometric representation, logically dividing the geometric representation within the boundary into a plurality of regions, and analyzing at least one feature for each of the plurality of regions within the boundary.

  • Genetic Data Analysis and Database Tools

    A computerized tool and method for delivery of pharmacogenetic and pharmacological information, comprising a core system having algorithms and databases for storing, collating, accessing, cross-referencing, and interpreting genetic and pharmacologic data, with a graphical user interface for a client network of providers of laboratory genetic testing services to access the core services under contract. The system includes "paypoints" in support of improved business models. Included are mechanisms for `pass through` third party and insurance reimbursement for interpretive reports, insurance reimbursement for on-line access to pharmacogenetic information at the point of care, tools for market segmentation, and a conversion tool for capturing new subscribers. Also disclosed are tools and predictive algorithms for preventing drug-drug and drug-gene adverse drug reactions.

  • METHODS OF SELECTION, REPORTING AND ANALYSIS OF GENETIC MARKERS USING BROAD-BASED GENETIC PROFILING APPLICATIONS

    Disclosed is a method for determining whether an individual has an enhanced, diminished, or average probability of exhibiting one or more phenotypic attributes and related methods of selecting a set of genetic markers; for providing relevant genetic information to an individual; of evaluating the probability that progeny of two individuals of the opposite sex will exhibit one or more phenotypic attributes; and for determining the genomic ethnicity of an individual.

  • METHODS OF SELECTION, REPORTING AND ANALYSIS OF GENETIC MARKERS USING BROAD-BASED GENETIC PROFILING APPLICATIONS

    Disclosed is a method for determining whether an individual has an enhanced, diminished, or average probability of exhibiting one or more phenotypic attributes and related methods of selecting a set of genetic markers; for providing relevant genetic information to an individual; of evaluating the probability that progeny of two individuals of the opposite sex will exhibit one or more phenotypic attributes; and for determining the genomic ethnicity of an individual.

  • RCA LOCUS ANALYSIS TO ASSESS SUSCEPTIBILITY TO AMD AND MPGNII

    The invention relates to gene polymorphisms and genetic profiles associated with an elevated or a reduced risk of alternative complement cascade deregulation disease such as AMD and/or MPGNII. The invention provides methods and reagents for determination of risk, diagnosis and treatment of such diseases. In an embodiment, the present invention provides methods and reagents for determining sequence variants in the genome of a individual which facilitate assessment of risk for developing such diseases.

  • Quality Assurance Methods for Medication Therapy Management

    A method for improving quality assurance methods for medication therapy management using a computer particularly adapted for a health care management or delivery organization is provided, the method including at least the steps of: providing a set of internal controls, wherein the internal controls further include a set of national guidelines and standards, as well as a qualified staff; and development by the qualified staff of a clinical rule based upon the national guidelines and standards. In other embodiments, a clinical quality assurance team further reviews the clinical rule developed by the staff. In further embodiments, a set of external controls is provided, wherein the external controls include one or more of a plurality of approved pharmacists, an external clinical advisory council, and/or an independent review board.

  • METHODS AND PRODUCTS RELATED TO THE IMPROVED ANALYSIS OF CARBOHYDRATES

    The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information from the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation. Such methods are also provided. Methods are further provided to assess production processes, to assess the purity of samples containing glycoconjugates, and to select glycoconjugates with the desired glycosylation.

  • Data word analysis by spectroscopy

    Multisource fusion of data is used on a handheld device to create a portable analytical verifier, including the ability to verify based on product wrapping or using a re-turned code to check the product or wrapping. Data words are extracted from authentication media by testing to see whether particular taggants are present, using spectroscopic analysis.

  • Data word analysis by spectroscopy

    Multisource fusion of data is used on a handheld device to create a portable analytical verifier, including the ability to verify based on product wrapping or using a re-turned code to check the product or wrapping. Data words are extracted from authentication media by testing to see whether particular taggants are present, using spectroscopic analysis.

  • PROCESS FOR THE AUTOMATED CLASSIFICATION AND ANALYSIS OF RAW DATA AND ITS USE IN THE OPTIMIZATION OF PROCESSES

    The present invention relates to a process for the automated classification and analysis of data, said process comprising the steps of i) selecting an analytical method for use in an analytical experiment; ii) determining working parameters and conditions for the selected method, which parameters and conditions have an effect on the results obtained by using the selected method; iii) assigning to a set of data, said set of data including a) the type of the selected method and b) a specific combination of working parameters and conditions used in the analytical experiment a unique analytical method code (Hash value); iv) applying the method characterized by said Hash value to at least one reference sample and at least one or more further samples obtained from a process for preparing said sample, in which process at least one parameter such as temperature, solvent, or ratio of reactants etc. is to be optimized; v) storing the Hash value and the name of the sample as metadata together with the analytical results obtained in step (iv) as an analytical report; vi) automatically extracting, e.g. by using an extraction template or another extraction process, the relevant metadata from the analytical reports and transferring them to an enterprise content management system (ECMS) or other database systems; vii) automatically organizing the results according to the Hash value in the ECMS or any other suitable database system, viii) correlating the sets of data characterized by different Hash values on the basis of the reference sample measured for each Hash value, thereby creating a correlation matrix/table; ix) using the correlation matrix/table for multivariate analysis of the one or more of the parameters of the process resulting in the samples recited in step (iv); x) optionally, visualizing the results as one or more graphic charts; xi) using the results of steps (ix) and, optionally, (x) to optimize said one or more parameters of the process as defined in step (iv); xii) optionally, verifying the process on the laboratory, pilot plant or industrial scale.

  • Method for quantitative analysis of complex proteomic data

    This invention is a novel method for analysis of data that is produced by test equipment. The preferred embodiment is data produced by liquid chromatography tandem mass spectrometry (LC-MS/MS) equipment, using industry standard methods to generate the initial data from the test equipment. The invention is a method for processing of the data to promptly produce accurate, reliable, and meaningful data that can be used for critical decisions. The unique benefit of the method is to correct the multiple measurement and calculation errors that are inherent in the operation of laboratory equipment. Prior methods result in errors based on circumstances that are difficult to control, accuracy-related errors in machine measurements, and fundamental mathematical errors in the data processing software that used with the laboratory equipment. As an added benefit, this novel method allows comprehensive simultaneous measurement and calculation of correlation of any and all peptide pairs in a single measurement, with the capability to support repeated measurements with changed conditions over time. This novel method allows robust, detailed, and comprehensive measurements of peptide activity and function, which results in substantial improvements over prior methods in accuracy, reliability, and efficiency.

  • Apparatus And Method For Coupled LC-NMR Analysis

    A device for performing chromatographic separations and nuclear magnetic resonance analysis has trapping means for holding a separated sample and to form a held separated sample and placing said held separated sample in said nuclear magnetic resonance assembly. One preferred trapping means forms a held separated sample and a passed separated sample. The passed separated sample is discharged from the device. Preferred trapping means comprise a trapping column or a separated sample loop.

  • PHARMACEUTICAL DATA INTEGRATION & ANALYSIS TOOL

    A system and processes are used to strategically evaluate opportunities for developing and producing generic pharmaceuticals. The system includes a knowledge driven optimization tool that efficiently analyzes pharmaceutical opportunities after merging pharmaceutical records from multiple sources. The analytical system allows users to filter the integrated records and evaluate the integrated records using patterns in the data. The system calculates historical trends of generic pharmaceutical compositions, and a user can select a market entry template that is based on the historical trends which the system then applies to a current pharmaceutical composition to create a forecast.

  • Techniques For Sample Analysis

    Techniques are described for performing sample analysis. Liquid chromatographic separation of a sample is performed and an eluent is generated. Mass spectrometry on said eluent is performed to detect a compound where the compound may occur in trace amounts. The compound may have a concentration, for example, of approximately less than one part per trillion.

  • CHROMATOGRAPHY COLUMN AND MAINTENANCE METHOD

    A method of maintenance of a chromatography column is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. The provision of guide elements which can be reversibly attached to the column facilitates removal or insertion of column components.

  • CHROMATOGRAPHY DEVICES AND METHODS

    Provided are chromatography devices having a stationary phase that includes patchy particles having at least two different surface chemistries, such as Janus particles. Also provided are methods of separating at least one analyte out of a sample, where the method includes adding a sample having at least one analyte to a chromatography device that includes a stationary phase that includes a plurality of patchy particles. Further provided are methods that include packing a chromatography column with a plurality patchy particles having at least two different surface chemistries. Additional embodiments are disclosed.

  • MULTI-DIMENSIONAL CHROMATOGRAPHIC METHODS FOR SEPARATING N-GLYCANS

    A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.

  • Purification of Blood Coagulation Factors

    The present invention relates to the purification of vitamin K-dependent blood coagulation factors, such as Factor IX (FIX). In particular, the invention provides a method for purifying Factor IX having a desired content of gamma-carboxyglutamic acid from a sample comprising a mixture of species of said Factor IX having different contents of gamma-carboxyglutamic acid, said method comprising the steps of: (a) loading said Factor IX sample onto an immunoaffinity chromatography material coupled to a binding moiety for gamma-carboxyglutamic acid; (b) eluting said Factor IX; and (c) selecting a fraction obtained from said elution wherein the polypeptides in the fraction have the desired content of gamma-carboxyglutamic acids; characterised in that the total concentration of Factor IX within said sample exceeds the binding ability of the immunoaffinity chromatography material.

  • COMPUTER METHOD AND SYSTEM FOR PREDICTING PHYSICAL PROPERTIES USING A CONCEPTUAL SEGMENT MODEL

    Method of conducting chromatography comprising controlling a retention time of one or more chemical species in a mixture by determining at least one conceptual segment of: a) the one or more chemical species, b) a mobile phase component, and c) a stationary phase component. The method further includes defining an identity and an equivalent number of each of the at least one conceptual segment.

  • ANALYTICAL DEVICES FOR DETECTION OF LOW-QUALITY PHARMACEUTICALS

    An analytical device, in particular, a paper analytical device (PAD), for detection of at least two chemical components indicative of a low quality pharmaceutical product is provided. The analytical device can include a porous, hydrophilic medium, at least two assay regions associated with the porous, hydrophilic medium, at least one assay reagent or precursor thereof in the assay regions, and at least one electronically readable information zone.

  • METHOD AND SYSTEM FOR AUTOMATED QUALITY CONTROL PLATFORM

    A method and system for performing automated quality control analysis of a plurality of radiopharmaceuticals are provided. The method includes uniquely identifying each of the plurality of radiopharmaceuticals, and identifying and implementing a tailored quality control program for each of the radiopharmaceuticals. The method further includes automatically performing a plurality of selected quality control tests for each of the radiopharmaceuticals based on the tailored quality control program, and collecting information produced by the plurality of selected quality control tests.

  • ELECTROCHEMICAL DETECTION CELL FOR LIQUID CHROMATOGRAPHY SYSTEM

    A detection cell for a chromatography system includes a cell body having an inlet, an outlet, and a counter electrode, a working electrode, a sample flow passageway extending between the inlet and the outlet and in fluid contact with the counter and working electrodes, and a palladium/noble metal reference electrode system. A method of using the detection cell is also described.

  • METHOD FOR PERFORMING MAINTENANCE ON A CHROMATOGRAPHY COLUMN

    A method for performing maintenance on a chromatography column (1; 41) comprising the steps of: a) when maintenance is required, detaching a detachable joint (7; 63) between a bottom plate (5; 53) of the chromatography column and a stand (3; 43) on which the chromatography column is provided; b) attaching an adaptor (15; 59) provided in the chromatography system to any part of the chromatography column (1; 41) making it possible to lift the bottom plate (5; 53) from the stand (3; 43) with the adaptor (15; 59); and c) raising the adaptor (15; 59) and thereby also the bottom plate (5; 53) such that maintenance can be performed under the bottom plate (5; 53).

  • FILLER FOR AFFINITY CHROMATOGRAPHY

    Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

  • CHROMATOGRAPHIC KIT AND CHROMATOGRAPHY METHOD

    A chromatographic kit is provided including a labeling substance holding area having a labeling substance modified with a first binding substance of a test substance, and a labeling substance capturing area having a second binding substance of the test substance or a binding substance of the first binding substance in this order from upstream to downstream of a development direction of a test sample including the test substance, and further including an area having a color developing reagent in order to detect a first amplification reagent of two types of amplification reagents used to amplify the signal of the labeling substance when detecting the labeling substance.

  • METHODS AND SYSTEMS FOR ANALYSIS OF PEPTIDE SAMPLE STREAMS USING TANDEM MASS SPECTROSCOPY

    The present disclosure relates to methods and systems for analyzing a peptide sample stream from a chromatography column using tandem mass spectroscopy. Analysis of the sample stream during a first time interval is performed in order to identify peptides, such as tryptic peptides, that are contained in the sample stream. Database searching is then performed to identify one or more protein sequences that contain the identified peptide sequence and to identify associated peptide sequences that are contained in the protein sequence that differ from the peptide sequence. The retention time of associated peptides is estimated based on the hydrophobicity of the predicted peptides and by spiking the sample with standard peptides. Information on associated peptides can then be used to configure the mass spectrometer during a second time interval to detect or ignore ions that correspond to the associated peptides.

  • Quality Assurance Methods For Medication Therapy Management

    A method for improving quality assurance methods for medication therapy management using a computer particularly adapted for a health care management or delivery organization is provided, the method including at least the steps of: providing a set of internal controls, wherein the internal controls further include a set of national guidelines and standards, as well as a qualified staff; and development by the qualified staff of a clinical rule based upon the national guidelines and standards. In other embodiments, a clinical quality assurance team further reviews the clinical rule developed by the staff. In further embodiments, a set of external controls is provided, wherein the external controls include one or more of a plurality of approved pharmacists, an external clinical advisory council, and/or an independent review board.

  • CHROMATOGRAPHY COLUMN AND MAINTENANCE METHOD

    A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device.

  • Ultraviolet and High-Performance Liquid Chromatography Methods for the Evaluation of Sunscreen Efficacy

    Disclosed are compositions which can mimic DNA and/or RNA in cells of a subject and methods of using them as a substrate in testing efficacy of one or more compositions in reducing and/or preventing radiation, such as ultraviolet (UV) radiation-caused DNA and/or RNA damage of said subject. Also disclosed are systems related to the disclosed methods.

  • Chromatography Methods

    This application discloses, in part, 1) a stationary phase column and compression designs for preparative chromatography, 2) a method of improving performance of silica gel chromatography by controlling the hydration of silica gel and acidifying the mobile phase, and 3) a method of extending the life of a silica gel column packing by cleaning or regenerating the silica gel stationary phase.

  • LASER ABLATION ELECTROSPRAY IONIZATION (LAESI) FOR ATMOSPHERIC PRESSURE, IN VIVO, AND IMAGING MASS SPECTROMETRY

    The field of the invention is atmospheric pressure mass spectrometry (MS), and more specifically a process and apparatus which combine infrared laser ablation with electrospray ionization (ESI)

  • Nanomanipulation Coupled Nanospray Mass Spectrometry (NMS)

    A coupled nanomanipulation and nanospray mass spectrometry (NMS) system for single cell, single organelle, and ultra-trace molecular analysis is disclosed herein. The system primarily comprises a bio-workstation coupled to a NMS. The bio-workstation primarily comprises of a nanomanipulator stage with a plurality of nano-positioners attached to a cabinet with a piezo voltage source and a pressure injector. The present invention further describes a fingerprint lift method that when coupled with the system disclosed herein can be used for retrieval and analysis of trace amounts of drug and explosive residues. The system described herein has been used in the areas of trace and document analysis within the forensic field, trace fiber analysis, and electrostatic lifts for illicit drugs, as well as document and painting analysis.

  • ANTIBODY PURIFICATION VIA AFFINITY CHROMATOGRAPHY

    Embodiments herein provide methods of purifying monoclonal and polyclonal anti-bodies (e.g., immunoglobulins) from biological fluids, such as cell lysates, cell supernatant and ascites fluids, using small molecule affinity chromatography. Various embodiments disclose a class of small molecules that selectively bind a nucleotide binding site that is inherent to all immunoglobulins, and in various embodiments, methods are disclosed that use one of these small molecules as a capture molecule in small molecule affinity chromatography. In some embodiments, the small molecule may be an indole, and in particular embodiments, the small molecule may be indole-3-butyric acid.

  • METHOD AND APPARATUS FOR REACTION CHROMATOGRAPHY

    An apparatus for reaction chromatography comprising: a chromatography column, the column having a fluid outlet for an eluate flow, wherein the fluid outlet is configured with two or more fluid ports, the two or more fluid ports comprising one or more reactant ports, wherein each reactant port is for connecting a reactant flow into the eluate flow to react with the eluate flow, and one or more product ports for receiving the reacted eluate flow; one or more reactant sources in fluid communication with the one or more reactant ports to provide the reactant flow; and one or more processing units in fluid communication with the one or more product ports to process the reacted eluate flow.

  • PURIFICATION OF VACCINIA VIRUSES USING HYDROPHOBIC INTERACTION CHROMATOGRAPHY

    The present invention relates to methods for purification of Vaccinia viruses (VV) and/ or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.

  • ANALYZING TARGET ANALYTES IN A SAMPLE USING MASS SPECTROSCOPY

    A method for determining an amount of an analyte in a sample by mass spectrometry can include the steps of (i) ionizing an analyte from the sample and a deuterated analog of the analyte, to produce an analyte ion and a deuterated analog ion, where the deuterated analog undergoes fragmentation and deuterium scattering during mass spectrometry; (ii) measuring an analyte ion signal and a deuterated analog ion signal by mass spectrometry, where the deuterated analog ion signal is measured using a mass transition resulting from fragmentation and deuterium scattering; and (iii) determining an amount of analyte in the sample using the analyte ion signal and the deuterated analog ion signal. Corresponding kits can include instructions for carrying out the method, together with a deuterated analog of the analyte selected to undergo fragmentation and deuterium scattering during mass spectrometry and exhibit a mass transition resulting from fragmentation and deuterium scattering.

  • MULTISCALE LIGHT AMPLIFICATION STRUCTURES FOR SURFACE ENHANCED RAMAN SPECTROSCOPY

    A method, system and article of manufacture for amplification of light for surface enhanced Raman spectroscopy. The method and system include a source of input light, a grating with grooves therein, a nanoparticle array disposed in the grooves with the nanoparticles and grating having a variety of selectable parameters. The combination of the nanoparticles and selected characteristics, including generating hot spots, and the features of the grating enable enhanced amplification of the input light signal to provide an output Raman signal of greatly increased intensity for Raman spectroscopy.

  • SPECTROSCOPY SYSTEMS AND METHODS USING QUANTUM CASCADE LASER ARRAYS WITH LENSES

    A spectroscopy system includes an array of quantum cascade lasers (QCLs) that emits an array of non-coincident laser beams. A lens array coupled to the QCL array substantially collimates the laser beams, which propagate along parallel optical axes towards a sample. The beams remain substantially collimated over the lens array's working distance, but may diverge when propagating over longer distances. The collimated, parallel beams may be directed to/through the sample, which may be within a sample cell, flow cell, multipass spectroscopic absorption cell, or other suitable holder. Alternatively, the beams may be focused to a point on, near, or within the target using a telescope or other suitable optical element(s). When focused, however, the beams remain non-coincident; they simply intersect at the focal point. The target transmits, reflects, and/or scatters this incident light to a detector, which transduces the detected radiation into an electrical signal representative of the target's absorption or emission spectrum.

  • KARL FISCHER TITRATOR AND KARL FISCHER TITRATION METHOD

    In the invention, a back titration and titer determination can be made using a back coulometric titration. When the iodine exists in a solution put in the titration flask with electrolytic electrodes, a back coulometric titration is performed to produce iodine ions from the iodine at the anode of the electrolytic electrodes. Where the solution is a dehydrated solvent including a Karl Fischer reagent for a volumetric titration, the titer can be determined by the back coulometric titration. Where the solution is anolyte in which the iodine remains after the coulometric titration in the coulometric titration method, the water content in the sample is found from the quantity of electricity consumed by the coulometric titration and the quantity of electricity consumed by the back coulometric titration. Where the solution is anolyte in which the iodine remains by adding Karl Fischer reagent in the volumetric titration method, the water in the sample put in the titration flask is found from the added amount of Karl Fischer reagent and the quantity of electricity consumed by the back coulometric titration.

  • IMMUNOASSAY METHODS

    The application generally relates to the field of diagnostic or prognostic assays and in particular relates to assays for the detection of antibodies in a sample comprising patient bodily fluid, wherein such antibodies are used as biological markers of a disease state or disease susceptibility. The assay is based on cross-titration of both the patient bodily fluid to be tested for the antibody and an antigen used to detect the antibody by specific binding.