The strain was procured from National chemical laboratory, Pune. The fermentation conditions were studied: fermentation period 4 d, pH 6.0 and temperature 28°C. Under these conditions, this strain produced 15.23 g/l of pullulan. The parent strain was mutated by ultraviolet irradiation and the fermentation conditions were studied with the mutants. Isolated polymer was characterized by fourier transform infra-red (FTIR) and antimicrobial activity was also studied. Drug release behavior of the polymer was evaluated from the prepared microsphere using donepezil HCl. Microspheres were characterized by FTIR, differential scanning calorimetry (DSC), Scanning electron microscopy (SEM), Zeta potential, particle size and entrapment efficiency. The strain exposed under UV light of 25 minutes produced 22.06 g/l. FTIR spectrum is similar to that of standard pullulan. Isolated polymer of pullulan showed negative antimicrobial activity. Prepared microspheres showed almost similar drug release behavior to that of standard polymer. FTIR and DSC studies revealed that there is no interaction between polymer and drug. Prepared microspheres had particle size of 535.9 nm, zeta potential of −7.44 mV, entrapment efficiency 88.64% and cumulative drug release of 95.78% in 24 h.
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