The application of plant extracts for therapeutic purposes has been used in traditional\nmedicine since the plants are a source of a great variety of chemical compounds that possess\nbiological activity. Actually, the effect of these extracts on diseases such as cancer is being widely\nstudied. Colorectal adenocarcinoma is one of the main causes of cancer related to death and the\nsecond most prevalent carcinoma in Western countries. The aim of this work is to study the possible\neffect of two fenugreek (Trigonella foenum graecum) protein hydrolysates on treatment and\nprogression of colorectal cancer. Fenugreek proteins from seeds were hydrolysed by using two\nenzymes separately, which are named Purafect and Esperase, and were then tested on differentiated\nand undifferentiated human colonic adenocarcinoma Caco2/TC7 cells. Both hydrolysates did not\naffect the growth of differentiated cells, while they caused a decrease in undifferentiated cell\nproliferation by early apoptosis and cell cycle arrest in phase G1. This was triggered by a\nmitochondrial membrane permeabilization, cytochrome C release to cytoplasm, and caspase-3\nactivation. In addition, the hydrolysates of fenugreek proteins displayed antioxidant activity since\nthey reduce the intracellular levels of ROS. These findings suggest that fenugreek protein\nhydrolysates could be used as nutraceutical molecules in colorectal cancer treatment.
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