Fish is the source of polyunsaturated fatty acids, especially omega-3 fatty acids. Linolenic acid, Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) are omega-3 fatty acids. Omega 3 fatty acids have very much importance in the food industry. In the present work, fish oil was extracted using the supercritical CO2 method and compared with soxhlet extraction method in quality and yield. Supercritical fluid extraction conditions were optimized in terms of time, temperature and pressure with an objective of getting the quality of extracted oil and in a cost effective manner. The fatty acid composition of oils was determined by GC/MS analysis. From this study, it was concluded that Supercritical CO2 process is better than soxhlet method in terms of desired fatty acid extraction i.e. omega-6 and omega-3 fatty acids.
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