Background. Software guided optic nerve assessment can assist in process automation and reduce interobserver disagreement.We\ntested depth analysis software (DAS) in assessing optic nerve cup-to-disc ratio (VCD) from stereoscopic optic nerve images (SONI)\nof normal eyes. Methods. In a prospective study, simultaneous SONI from normal subjects were collected during telemedicine\nscreenings using a Kowa 3Wx nonmydriatic simultaneous stereoscopic retinal camera (Tokyo, Japan). VCD was determined from\nSONI pairs and proprietary pixelDAS (Kowa Inc., Tokyo, Japan) after disc and cup contour line placement. A nonstereoscopicVCD\nwas determined using the right channel of a stereo pair. Mean, standard deviation,
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