Tick populations are controlled through the application of chemical pesticides. However,\nthe rise in chemical resistance has prompted the investigation of other control methods such as the\nuse of tick vaccines. Proteomic analysis provides valuable information about the possible function\nand localization of proteins, as candidate vaccine proteins are often either secreted or localized\non the cell-surface membrane. Progress in the utilization of proteomics for the identification of\nnovel treatment targets has been significant. However, their use in tick-specific investigations is still\nquite novel, with the continual development of tick-specific methodologies essential. In this study,\nan innovative sample preparation method was utilized to isolate epithelial cells from tick midguts to\nidentify the membrane-bound proteins. Proteomic analysis was conducted comparing crude and\ninnovative sample preparation methods with 692 and 1242 tick-specific proteins, 108 and 314 surface\nproteins respectively, isolated from the midguts of semi-engorged Rhipicephalus microplus adult female\nticks. This research reports a novel preparation protocol for the analysis of tick midgut proteins which\nreduces host protein contamination.
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