Natural radical scavengers have recently received considerable interest owing to the role of free radicals in causing oxidative stress in living organisms. Flavonoids constitute one of the most important families of molecules with antioxidant activities, a characteristic associated with the presence in their structure of hydroxyl groups bound to aromatic rings. The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Givotia rottleriformis bark. Three flavonoids were isolated viz., Rutin (I), Kaempferol 3-O-[2-O-(6-O-feruloyl)-β-D-glucopyranosyl]-β-D-galactopyranoside (II) and Luteolin-7-O-β-D-Glucuronide (III). A comprehensive study on the phytochemical contents-total phenolic content and flavonoid content was accessed using Folin-ciocalteu and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 13.80 % w/w and 5.7 % w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 230 µg/mL, 220 µg/mL and 180 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: rutin (IC50 values 18, 15, 16 µg/mL) >luteolin (IC50 values 22, 20, 18 µg/mL) > kaempferol (IC50 values 25, 28, 24 µg/mL) respectively.
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