Objective:The objective of the present study was to evaluate CNS depressant, anxiolytic and nootropic activity of ayurvedic formulation “Dhasara” of Acorus calamus root in mice. Material and Methods: Acorus calamus root was collected, dried and ayurvedic formulation Dhasara of Acorus calamus (DHAC) was prepared freshly at each time. Female swiss albino mice weighing between 20-25 gm were used. CNS depressant activity of DHAC (100 mg/kg, p.o.) was measured by Open field behavior model using diazepam (2 mg/kg, p.o) as a reference standard. Anxiolytic activity of DHAC (100 mg/kg, p.o.) was evaluated by Elevated plus maze (EPM) model using diazepam (2 mg/kg, p.o.) as a reference standard. Nootropic activity of DHAC (100 mg/kg, p.o.) was evaluated by Elevated plus maze (EPM) model using piracetam (150 mg/kg, p.o.) as a reference standard and on third-day Scopolamine (3 mg/kg, i.p.) was given to induce amnesia. Result: In open field model, DHAC showed significant reduction in number of square crossed and number of rearing as compared to control group. In Elevated plus maze model, DHAC showed significant increase in time spent in open arm as compared to control group and for nootropic activity DHAC increase the latency period for entry into close arm as compared to control group. It also decrease latency period after giving scopolamine. Conclusion:In short, Ayurvedic formulation “Dhasara” of Acorus calamus (DHAC) shows CNS depressant, anxiolytic and nootropic activity in mice.
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