Present study was undertaken to explore the interaction potential of Glycyrrhiza glabra with Adhatoda vasica on antimicrobial activity. To derive possible magnitude of interaction at various combinations, Checkerboard microtitre test was performed and Fractional Inhibitory Concentration Combination Index values (FICI) were used to analyze and compare the different combination effects between the herbs tested.The results showed that antibacterial effects of 80% ethanolic extract both of both herbs against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus were concentration dependant. Both the extracts showed significant (P< 0.01) antibacterial activity as compared to control. In present study two-fold serial dilution technique was used to determine the minimal inhibitory concentration (MIC) of Adhatoda vasica extract (AVE) and Glycyrrhiza glabra extract (LE) against the selected bacterial strains. The results revealed that the AVE have antibacterial effects against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus at concentration levels of 750, 1000, 750, 1000 and 1000 µg/ml, respectively. While LE have antibacterial effects against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus at concentration levels of 250, 500, 125, 500 and 250µg/ml, respectively. Results of checkerboard assays suggested that MIC of LE combined with AVE was remarkably decreased. Moreover, Bacterial susceptibility to AVE and LE was enormously improved by combination. FIC indices indicated the synergism between AVE and LE against S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus, with an FIC index of 0.467, 0.5, 0.467, 0.5 and 0.425 respectively. In conclusion, we suggest the combination of AVE and LE for the treatment of S abony, E. coli, B. Pumulus, P.aeruginosa, M. luteus may reduce the efficacious dose of AVE and LE alone and thus minimize the side-effects. Further in vivo experiments are necessary to assess the potential for therapeutic application.
Loading....