The present study was aimed at Standardization of the leaves of Stevia rebaudiana Bertoni & it’s herbal formulation (Bio-sweet tablet). For standardization, physicochemical parameters were measured for the plant & formulation. The phytomarker-Stevioside have been isolated by developed isolation method from the leaves of Stevia rebaudiana & identified by spectral data. Also estimation of stevioside in plant & formulation was carried out using HPTLC.
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