A simple, economic, selective and precise stability-indicating RP-HPLC method has been developed and validated for analysis of Beclomethasone dipropionate(BD),an anti inflammatory, in pharmaceutical dosage form. Reversed-phase chromatography was performed on a ACE C18, 5μ, 15 cm × 4.6 mm column with ACN–water, 60:40 (%, v/v), as mobile phase at a flow rate of 1.0 ml/min. Detection was performed at 238 nm .The drug was subjected oxidation, hydrolysis, heat and light to apply stress conditions. The drug was found to be hydrolyzed in acidic and alkaline conditions. We had also found the degradation under photolytic stress condition. The developed method was able to separate all degradation product generated under forced degradation studies and drug peak was eluted at 7.9 min. The developed method was validated as per ICH guideline for the parameters such as specificity, linearity, precision, accuracy, limit of detection, limit of quantification and found to be satisfactorily. Linear regression analysis data for the calibration plot showed there was a good linear relationship between response and concentration in the range 2-16μg/ml the regression coefficient was 0.9998 and the linear regression equation was y = 12413x + 1979.2. The detection (LOD) and quantification (LOQ) limits were 0.039 and 0.12μg/ml respectively.
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