Tramadol Hydrochloride and Diclofenac Sodium are used in combined dosage form for used for the symptomatic treatment of moderate to severe pain in adult patients. Present work sconsist development and validation of Ratioderivative UV Spectrophotometric for simultaneous estimation of Tramadol Hydrochloride (TRA) and Diclofenac Sodium (DCS) in Synthetic mixture. These UV spectroscopy methods are developed using Shimadzu double beam UV visible spectrophotometer (Model 1800) loaded with UV Probe 2.32 software. For ratio first derivative wavelengths were selected 230 nm for TRA and 245.2 nm for DCS Methanol used as a solvent. In this UV spectrophotometric methods linearity of TRA and DCS were found to be in concentration range of 4-24 µg/mL and 6-36 µg/mL respectively with correlation coefficient >0.998. This method is simple, accurate, precise, economical and statistically validated. There was no interference of any excipient in the determination of drugs in synthetic mixture. These method can be applied for routine quality control analysis and dissolution study.
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