A Simple, Sensitive and precise reversed-phase High performance liquid chromatography method was developed and validated for the simultaneous determination of Losartan Potassium(LSK) and Atenolol (ATN) in tablet. The separation was achieved on RP C-18 (250 mm x 4.6 mm, 5 μm), using Methanol and Water (42:58 v/v) as mobile phase at a flow rate of 1.0 ml/min. Detection was carried out using a UV detector at 226 nm. The method was validated. The developed and validated method was successfully applied for the quantitative analysis of Losar beta® tablet. The Calibration curves were linear over the ranges of 8-18 µg/ml for Losartan Potassium and Atenolol both. The retention time for Losartan Potassium and Atenolol was found to be 2.70 and 4.91 min, respectively. The percentage recovery was found to be 99.94% w/w and 100% w/w for Losartan Potassium and Atenolol, respectively. The values obtained of LODs were 0.01859 and 0.02882 µg/ml and LOQs were 0.05633 and 0.08732 µg/ml for Losartan Potassium and Atenolol, respectively. The proposed method is rapid, precise and accurate for the determination of Losartan Potassium and Atenolol for routine quality control of tablet containing these two drugs.
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