A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Amitriptyline HCl (AMI) and Chlordiazepoxide (CHLOR) by employing first order derivative zero crossing method in 0.1 N HCl. The first order derivative absorption at 239 nm (zero cross point of Amitryptiline HCl) was used for quantification of Chlordiazepoxide and 246 nm (zero cross point of Chlordiazepoxide) for quantification of Amitriptyline HCl. The linearity was established over the concentration range of 5-10 µg/ml and 6-16 µg/ml for Chlordiazepoxide and Amitriptyline HCl with correlation coefficient r2 0.9900 and 0.9983, respectively. The mean % recoveries were found to be in the range of 99.63%–100.27% and 98.89%–100.72% for Chlordiazepoxide and Amitriptyline HCl, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of pharmaceutical dosage form.
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