A simple, economical, precise, and accurate zero crossing difference spectrophotometric method for simultaneous determination of cefadroxil and probenecids in synthetic mixture have been developed. Measurements of absorbance were carried out at zero-crossing wavelengths 313 nm and 232 nm for cefadroxil and probenecid by zero-crossing difference spectrophotometric method. Beer’s law is obeyed in the concentration range of 4-36 μg/ml for cefadroxil and 5-25 μg/ml for probenecid by zero-crossing difference spectrophotometric method. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found.
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