The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective dual wavelength spectrophotometric method for the simultaneous determination of Domperidone and Cinnarizine in combined dosage form. The method was based on determination of Cinnarizine at 301.2 nm (λ1) and 248.2 nm (λ2) and Domperidone at 239 nm (λ3) and 258.2 nm (λ4). The difference in absorbance at λ1 and λ2 (A248.2–A301.2) cancels out the contribution of absorbance of Domperidone in measurement of Cinnarizine and the difference in the absorbance was proportional to the concentration of Cinnarizine in the combined mixture. While the difference in absorbance at λ4 and λ3 (A239–A258.2) cancels out the contribution of absorbance of Cinnarizine in measurement of Domperidone and the difference in the absorbance was proportional to the concentration of Domperidone in the combined mixture. The linearity was obtained in the concentration range of 10-40 μg/ml for Domperidone and 3-18 μg/ml for Cinnarizine. LOD and LOQ values were found to be 0.22 μg/ml and 0.11 μg/ml; 0.66 μg/ml and 0.35 μg/ml for Domperidone and Cinnarizine, respectively. Percent label claim of the compounds were 99.11±0.989 and 100.55±0.987 for Domperidone and Cinnarizine, respectively. Recovery data were found to be 99.86±0.65 for Domperidone and 99.45±0.50 for Cinnarizine. The method was successfully applied to pharmaceutical dosage form because no interference from excipients were found. The suitability of these methods for the quantitative determination of Domperidone and Cinnarizine was proved by validation. The proposed methods were found to be simple and sensitive for the routine quality control application of Domperidone and Cinnarizine in pharmaceutical dosage form. The results of analysis have been validated statistically and by recovery studies.
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