A simple, sensitive, rapid, accurate and precise spectrophotometric method was developed for simultaneous estimation of citicoline and methylcobalamin in tablet by employing absorption correction method and validated for accuracy, precision, linearity, LOD, LOQ. The wavelengths selected for quantitation were 271.0 nm for citicoline and 351.0 nm for methylcobalamin. Linearity was maintained within a wide concentration range from 5.0-25.0 μg/ml for citicoline and 10-50 μg/ml for methylcobalamin. The limit of detection and limit of quantification for citicoline were found to be 0.2335 and 0.7076 μg/ml respectively and for methylcobalamin 0.4819 and 1.4604 μg/ ml respectively. The method gives results of high accuracy confirmed by recovery studies. The mean % labeled claim for citicoline and methylcobalamin were 100.34±0.82% and 99.78±1.21% respectively. % R.S.D. values for precision studies were less than 2% indicates method is precise.
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