A simple, accurate, and precise dual wavelength spectrophotometric method was developed for simultaneous determination of Montelukast sodium and Olopatadine hydrochloride in synthetic mixture. The method is based on the principle that “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The method involves determination of Montelukast at 232.0 nm and Olopatadine at 206.0 nm. Both drugs follow Beer-Lambert’s law over the concentration range of 5-25 μg/ml and 2-10 μg/ml for Montelukast and Olopatadine, respectively. The % assay of the drugs was found near to 100% representing the accuracy of the method. The recovery of the Montelukast and Olopatadine were found near to 100%. Validation of the proposed method was carried out for its accuracy, precision, specificity and ruggedness according to ICH guidelines. Assay results for both drugs obtained by newly developed method were statistically compared with the reported methods for respective drugs which establish the reliability of the proposed method. The proposed methods can be successfully applied in routine work for determination of Montelukast and Olopatadine in synthetic mixture.
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