A fixed dose of Valacyclovir is used in tablet dosage form for the treatment and prevention of infectious diseases caused by Herpes Zoster. A simple, precise and accurate isocratic RP-HPLC method was developed and validated for the determination of Valacyclovir in tablet dosage form. As per ICH guideline Q1A (R2), drug was subjected different stress condition such as hydrolysis (acidic and alkaline), oxidation (3% H2O2 v/v), photolytic and thermal degradation. All stressed samples were successfully analyzed on an ACE C18 column (150×4.6mm id, 5µm particle size) using the mobile phase 10 mM potassium di-hydrogen phosphate buffer: Methanol (80:20 v/v, pH 6.5) at a flow rate of 1.0 mL/min. The retention time of Valacyclovir was found to be 3.879 min. The detection was performed using PDA detector or 252 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability and specificity. The method was linear in the concentration range of 20-140 µg/mL for with a correlation coefficient of 0.999. The accuracy (%recovery) was found to be in the range of 98.89-101.98%. It was found that drug was more degraded in acid, alkali and oxidative condition, while no prominent degradation was found in thermal and photolytic condition.
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