Isocratic, reversed phase liquid chromatographic assay method was developed for the quantitative determination of ibuprofen and phenylephrine hydrochloride in combined pharmaceutical dosage form. A Sunfire C18, 5 m column with mobile phase containing acetonitrile: methanol: phosphate buffer (50:20:30, v/v/v) (pH 6) adjusted with 0.01% O- phosphoric acid was used. The flow rate was 1.0 mL/min and effluents were monitored at 220 nm. The retention times of ibuprofen and phenylephrine hydrochloride were 13.6 min and 2.7 min, respectively. The linearity for ibuprofen and phenylephrine hydrochloride was in the range of 25-125 µg/ ml and 1.25–6.25 µg/ml, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. The method was successfully applied to the estimation of ibuprofen and phenylephrine hydrochloride in combined pharmaceutical dosage form.
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