This paper presents a RP-HPLC method for the simultaneous estimation of Dicyclomine and Tramadol Hydrochloride in combined tablet dosage form. The process was carried out on Sunshell Fused Core Shell C18 ( 100 x 4.6 mm), 2.6 μ column using phosphate buffer and Acetonitrile pH 7.5 in the ratio of 70:30 v/v respectively as a mobile phase at a flow rate of 1.5 mL/min. Detection wavelength was fixed at 224 nm. Linearity was observed in the concentration range of 4-20 μg/ml for Dicyclomine (r2=0.998) and 20-100 μg/ml for Tramadol Hydrochloride (r2=0.998). The retention time of Dicyclomine and Tramadol Hydrochloride was found to be 1.53 and 1.10 minutes, respectively. The developed method is simple, precise, specific, robust, rapid, reproducible, and sensitive and it can be used for estimation of Dicyclomine and Tramadol Hydrochloride in combined tablet dosage form.
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