UV Spectrophotometric method has been developed for simultaneous estimation of Dicyclomine (DCY) and Tramadol Hydrochloride (TRA) in bulk drug and in laboratory mixture. This method utilizes 0.1 N NaOH as a solvent and λmax of Dicyclomine and Tramadol Hydrochloride selected for analysis was found to be 224 nm and 276 nm respectively. Linearity was observed in the concentration range of 2.5-15 μg/ml for Dicyclomine (r2=0.997) and 12.5-75 μg/ml for Tramadol Hydrochloride (r2=0.996). The method was validated statistically and by recovery studies. The mean % recovery was 99.22-100.27% and 98.94-100.33% for Dicyclomine and Tramadol Hydrochloride respectively. Developed method was applied to laboratory mixture. The results were found to be within acceptance criteria according to ICH guideline. This method was simple, rapid, accurate and sensitive.
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