A simple, precise, accurate, economic and reproducible spectrophotometric method has been developed for simultaneous estimation of Montelukast Sodium (MONTE) and Olopatadine Hydrochloride (OLO) by employing Ratio first derivative UV spectrophotometric method in 0.1 N NaOH. The Ratio Spectra of Montelukast Sodium were obtained by divisor of Olopatadine Hydrochloride (20 µg/ml) and Ratio Spectra of Olopatadine Hydrochloride were obtained by divisor of Montelukast Sodium (40 µg/ml) and resulting ratio spectra was converted to first order derivative spectra. Wavelength Selected for detection of Montelukast Sodium 268.40 nm and for Olopatadine Hydrochloride 289.80 nm. The linearity was established over the concentration range of 20-40 µg/ml and 10-20 µg/ml for Montelukast Sodium and Olopatadine Hydrochloride with correlation coefficient 0.999 and 0.998, respectively. The mean % recoveries were found to be in the range of 99.77–100.31% and 98.33–100.07% for Montelukast Sodium and Olopatadine Hydrochloride respectively. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of Montelukast Sodium and Olopatadine Hydrochloride in their Synthetic mixture.
Loading....