A simple, efficient, accurate, precise and economical first order derivative spectrophotometric method was developed for the simultaneous determination of Xylometazoline hydrochloride and Ipratropium bromide in pharmaceutical dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra were obtained in distilled water and the determinations were made at 264.41 nm (ZCP of Xylometazoline hydrochloride) for Ipratropium bromide and 261.58 nm (ZCP of Ipratropium bromide) for Xylometazoline hydrochloride. The linearity was obtained in concentration range 250-875 µg/ml for Xylometazoline hydrochloride and 300-1050 µg/ml for Ipratropium bromide. The accuracy of the method was determined by recovery studies and was found to be in the range of 99.73-100.35% and 99.87-100.26% for Xylometazoline hydrochloride and Ipratropium bromide, respectively. The method was validated as per ICH guidelines and statically. The method showed good recovery with % RSD less than 2. The method was found to be simple, economic, accurate and precise and can be used routine analysis of Xylometazoline hydrochloride and Ipratropium bromide in pharmaceutical dosage form. The results of analysis have been validated statistically and by recovery studies.
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