A simple, rapid, accurate and precise first order derivative spectrophotometric method has been developed for simultaneous estimation of Cefpodoxime proxetil and Azithromycin Dihydrate in their combined pharmaceutical dosage form. This method utilizes methanol as a solvent. Zero crossing point (ZCP) of Cefpodoxime Proxetil and Azithromycin Dihydrate were found to be 234 nm and 283 nm respectively. Hence, estimation of Cefpodoxime Proxetil and Azithromycin Dihydrate were done at 283 nm and 234 nm respectively. Linearity was observed in the concentration range of 5-25 μg/ml for Cefpodoxime Proxetil (r2=0.9955) and 20-100 μg/ml for Azithromycin Dihydrate (r2=0.9968). The results of analysis have been validated statistically and by recovery studies. Developed method was applied to pharmaceutical dosage form. The results were found to be within acceptance criteria according to ICH guideline.
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