Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous estimation of Paracetamol (PCM) and Flupirtine meleate (FLU) in combined dosage form. Method A, Absorbance Correction Method, involves measurment at 320.00 nm (Corrected wavelenth) and 249.80 nm (λmax of Paracetamol) in zero order spectra. Method B, Ratio Derivative Method, involves division of spectra of Paracetamol by one selected standard spectrum of Flupirtine maleate and then measuring absorbance at 295.25 nm in ratio derivative spectra for estimation of Paracetamol. Similarly, spectra of Flupirtine maleate are divided by one selected standard spectrum of Paracetamol and then measuring absorbance at 297.60 nm in ratio derivative spectra for estimation of Flupirtine maleate. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 2.0-25.0 μg/mL for both drug with R square value greater than 0.999. Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. The methods were successfully applied for estimation of Paracetamol and Flupirtine meleate in marketed formulation.
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