UV spectrophotometric Dual wavelength method has been developed for simultaneous estimation of Diazepam and Imipramine Hydrochloride. The method involved measurement of absorbance difference at 267.2 nm and 304.0 nm (where Imipramine Hydrochloride have same absorbance) and absorbance difference at 235.6 nm and 278.0 nm (where Diazepam have same absorbance) of Diazepam and Imipramine Hydrochloride respectively. Beer’s law obeyed in concentration range of 3-15 µg/ml for Diazepam and 5-25 µg/ml for Imipramine Hydrochloride in 0.1 M HCl. The percent assay of marketed formulation was 100.30±0.53 and 100.45±0.71 of Diazepam and Imipramine Hydrochloride respectively. The proposed methods are rapid, simple, accurate and precise since they are recommended for routine analysis.
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