UV spectrophotometric Area under curve method has been developed for simultaneous estimation of Diazepam and Imipramine Hydrochloride. The method involved measurement of area at 279-289 nm (Diazepam) and at 245-255.0 nm (Imipramine Hydrochloride) of Diazepam and Imipramine Hydrochloride respectively. Beer’s law obeyed in concentration range of 3-15 µg/ml for Diazepam and 5-25 µg/ml for Imipramine Hydrochloride in 0.1 M HCl. The percent assay of marketed formulation was 100.30±0.53 and 100.45±0.71 of Diazepam and Imipramine Hydrochloride respectively. The proposed methods are rapid, simple, accurate and precise since they are recommended for routine analysis.
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