A new simple, sensitive high performance Liquid chromatographic (HPLC) method has been developed for the quantitative estimation of Levosulpride (LEVO) and Pantoprazole Sodium (PANTO) in combined dosage form using Hyperchrom ODS-BP C18250 x 4.6 mm i.d., (5 mm) was used as Stationary Phase. Acetonitrile: 0.05 M Potassium Dihydrogen Ortho Phosphate (50:50 v/v) (pH 3.0 adjusted by O-phosphoric acid) as mobile phase. LEVO and PANTO showed Rt value 3.344+0.005 and 4.753+ 0.006 and scanned at 288 nm. The method was validated in terms of linearity 38–114 μg/ml for LEVO and 20–60 μg/ml for LEVO. The limit of detection for LEVO and PANTO were found to be 1.0178 μg/ml and 0.5481 μg/ml, respectively and limit of quantification for LEVO and PANTO were found to be 3.0818 μg/ml and 1.660 μg/ml, respectively. The mean recovery was 98.32–101.00% and 98.50–101.85 % for LEVO and PANTO respectively. The method was found to be simple, sensitive, specific, accurate and precise and can be used for the routine quality control testing of Levosulpride and Pantoprazole Sodium dosage form.
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