Two simple Chemometric assisted UV-Spectrophotometric methods; Classical Least Square (CLS) and Inverse Least Square (ILS) were developed for simultaneous estimation of Paracetamol (PCM) and Flupirtine maleate (FLU) in combined dosage form without any chemical separation and any graphical treatment of the overlapping spectra of two drugs. The UV absorption spectra of the drugs studied, in the range of 250–320 nm, showed a considerable degree of spectral overlapping. Beer’s law was obeyed for both drugs in the concentration ranges of 2–25 µg/mL. Fourteen mixed solutions were prepared for the chemometric calibration as training set and seven mixed solutions were prepared as validation set. The absorbance data matrix was obtained by measuring the absorbance at thirty six wavelength points, from 250 to 320 nm with the interval of 2 nm (Δ=2 nm). The developed calibrations were successfully tested for pharmaceutical formulation.
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