Ceftazidime pentahydrate (CEF-β lactam antibiotic) is used for the treatment of resistant lower respiratory tract and other infections. An attempt was done to develop simple precise, accurate and validated HPTLC method for the estimation of CEF in bulk and injectable dosage form. The compounds were separated on aluminium-backed silica gel 60 F254 plates with Methanol: Toluene: Water: TEA (7.5:2:0.5:0.2 v/v/v/v) as mobile phase. These systems were found to give sharp spots of CEF with the Rf value of 0.44. Densitometric analysis of CEF was done at 254 nm. Regression analysis for the calibration plot was indicative of good linearity between response and concentration over the range 1250-7500 ng/band with the correlation coefficient (r2) 0.9972. The mean recoveries obtained for CEF was found to be 99.27%-100.15%. Statistical analysis proved that method is repeatable, selective, and accurate for estimation of CEF.
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