A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Meclizine and Caffeine from pharmaceutical formulation. The method was carried out on a C18 (25 cm x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of Methanol and water in the ratio of 70:30 v/v. The retention time of Meclizine and Caffeine was 3.3 min and 4.3 min respectively with the flow rate of 1mL/min. Eluents were detected at 230 nm. The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for Meclizine and Caffeine were R2=0.9990 and R2=0.9999 in the concentration range of 10-60µg mL-1 , 8-48 µg. mL-1 respectively. The relative standard deviation for intra-day precision was lower than 2%. The method was validated according to the ICH guidelines. The method was also found to be robust. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can be used for the e0.stimation of these drugs in combined dosage forms.
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