A simple, specific, accurate and economical uv-spectrophotometric method has been developed for estimation of niacin from bulk and pharmaceutical formulation. The solvent used to develop the method was methanol. The λ max of niacin in methanol was found to be 260 nm. The drug follows linearity in the concentration range of 2-10 µg/ml with correlation coefficient value 0.999. The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 99.99% was found in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels i.e. 25%, 50% and 100%.The % recovery was found to be in the range 98.88%-99.99%. The low values of % R.S.D are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The % R.S.D value less than 2 indicate that the method is precise. The above method was a rapid and cost-effective quality-control tool for routine analysis of niacin in bulk and in pharmaceutical dosage form.
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