A new simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of levosulpiride (LEVO) and esomeprazole magnesium trihydarte (ESO) in combined pharmaceutical formulation was developed. The spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectrums were obtained in methanol and the determinations were made at 302.58 nm (ZCP of esomeprazole) for quantification of levosulpiride and 320.17 nm (ZCP of levosulpiride) for quantification of esomeprazole. The linearity was obtained in the concentration range of 10-40 μg/ml for levosulpiride and 4-24 μg/ml for esomeprazole. The mean recovery was 100% – 101.7% and 98.33% – 99.2% for levosulpiride and esomeprazole respectively. The results of analysis have been validated statistically as per ICH guidelines.
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