A simple, precise and accurate method was developed for the estimation of chlorzoxazone (CHL) and paracetamol (PCM) in tablet dosage form using second order derivative spectrophotometry. Wavelengths selected for quantitation were 279.18 nm for chlorzoxazone (zero crossing point of PCM) and 270.43 nm for paracetamol (zero crossing point of CHL). The method was validated with respect to linearity, accuracy, precision, limit of detection and limit of quantitation in accordance with the international conference on harmonisation (ICH) guidelines. Linearity was observed in concentration range of 5-40 μg/ml for each chlorzoxazone and paracetamol. The limit of detection and limit of quantitation were found to be 0.16 μg/ml and 0.51 μg/ml for chlorzoxazone and 0.59 μg/ml and 1.83 μg/ml for paracetamol. The percentage recovery of chlorzoxazone and paracetamol was found to be 99.95±0.66 and 99.66±0.62 respectively. The % R.S.D. values for intra-day and inter-day precision study were < 2.0%, confirming that the method was precise. The method can be successfully employed for the simultaneous estimation of chlorzoxazone and paracetamol in tablet dosage form.
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