Two simple sensitive and accurate spectrophotometric methods for the determination of ciprofloxacin hydrochloride have been developed and validated. Method A engross the addition of a known excess of bromate-bromide mixture to ciprofloxacin hydrochloride in hydrochloric acid medium followed by determination of residual bromine by reacting with a fixed amount of thymol blue and measuring the absorbance at 545 nm. In method B ciprofloxacin hydrochloride, in presence of acidic medium reacts with excess amount of ceric ammonium sulphate and remaining reacts with crystal violet to produce coloured complex and the absorbance measured at 619 nm. The final stock solution was made to produce 25 µg/ml and 100 µg/ml with distilled water in method A and method B respectively. The linearity was found in concentration range of 2.5-12.5 µg/ml and 5-25 µg/ml for method A and method B respectively. The correlation coefficient was found 0.9994 and 0.9993 for method A and method B respectively. The regression equation was found as y=0.0672x-0.0035 and y= 0.0312x-0.0042 for method A and method B respectively. The method was validated according to USP and ICH Guidelines.
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