Two simple, sensitive and accurate spectrophotometric methods for the determination of norfloxacin hydrochloride have been developed and validated. The methods are based on the oxidative coupling reaction of norfloxacin hydrochloride with MBTH in the presence of Ce (IV) as an oxidant, to form a bluish green coloured product (method A), while norfloxacin hydrochloride in presence of acidic medium reacts with excess amount of chloramine-T and the unreacted chloramine-T reacts with crystal violet to produce greenish blue colour (method B). The final stock solution was made to produce 100 µg/ml with methanol for both method A and method B. The λmax was found to be 667 nm and 625 nm, for method A and method B respectively. The linearity was found in concentration range of 5-30 µg/ml and 10-50 µg/ml respectively for method A and method B. The correlation coefficient was found 0.999 and 0.9994 for method A and method B respectively. The regression equation was found as Y=0.0249x+0.0106 and Y=0.0153x+0.0086 respectively for method A and method B. The methods were validated according to ICH and USP guidelines.
Loading....