A simple, sensitive, specific, accurate and stability‑indicating reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of ebastine and phenylephrine hydrochloride, using a pursuit C18 (300 x 3.9 ) mm; 10 µm (particle size) column as a stationary phase and a mobile phase composed of methanol: 0.02 M ammonium acetate (60:40 v/v), pH 6.7. The retention times of phenylephrine hydrochloride and ebastine were found to be 5.11 min and 7.09 min, respectively. Linearity was established for ebastine and phenylephrine hydrochloride in the range of 4-34 μg/ml. The percentage recoveries of ebastine and phenylephrine hydrochloride were found to be in the range of 99.34-100.32% and 98.92-102.09%, respectively. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat and UV degradation. The degradation studies indicated ebastine and phenylephrine hydrochloride to be more susceptible to oxidative hydrolysis. This method can be successfully employed for simultaneous quantitative analysis of ebastine and phenylephrine hydrochloride and in bulk drugs and formulations.
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