Reverse phase-high performance liquid chromatography (RP-HPLC) method have been developed and validated for the estimation of allopurinol and α-lipoic acid in combination tablet. The developed method is rapid, accurate, precise, simple and economical. The separation was achieved on reversed-phase unisphere C18 column (agela tech.), (5 µm, 250 mm x 4.6 mm i.d) column using mobile phase consisting of acetonitrile: 0.05 M potassium dihydrogen phosphate (60:40, v/v) adjust pH with ortho phosphoric acid to 5.25 which gives greater resolution. Wavelength used for UV detection was 220 nm. Linearity was observed in the concentration range of 50-150 μg/ml for both allopurinol and α-lipoic acid with correlation coefficient 0.9999 and 0.9999 for allopurinol and α-lipoic acid respectively. The proposed method was successfully applied for the simultaneous estimation of both drugs in combination tablet. Assay value of allopurinol was found to be 100.90% and that of α-lipoic acid was found to be 100.11%. Mean recovery were found to be 100.75 % and 100.15 % for allopurinol and α-lipoic acid, respectively. LOD and LOQ were found to be 2.889 μg/ml and 8.755 μg/ml for allopurinol, respectively and 0.084 μg/ml and 0.255 μg/ml for α-lipoic acid, respectively. Proposed method can be successfully applied for the quantitative determination of Allopurinol and α-Lipoic Acid in combination tablet.
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