The main aim and focus of present study was to develop RP-HPLC method and validation in stability indicating manner as per ICH guidelines for the quantification of ibuprofen in pharmaceutical formulations. Ibuprofen is commonly used as non steroidal anti inflammatory drug (NSAID) to achieve higher pharmacological activity. The optimized chromatographic conditions includes a C-18 RP-HPLC column as stationary phase and the mobile phase pumped with the flow rate of 1.0 ml/min and the eluent was monitored at 220 nm were established. The mobile phase contains a combination of buffer and acetonitrile in the ratio of 40:60% (v/v). The developed method is a simple, rapid, precise, specific and accurate for the determination of ibuprofen in the range of 20% to 200% of working concentrations.
Loading....