Two simple, sensitive and accurate spectrophotometric methods for the determination of glibenclamide have been developed. The methods are based on diazotization reaction of glibenclamide with sodium nitrite for a specific time and later this diazotized product was coupled with aniline through heating which gives red coloured azo dye - method A, while in method B - colorimetric method was based on oxidation-reduction reaction. In this method ceric ammonium sulphate was made to react with glibenclamide in excess amount, in presence of sulphuric acid. The λmax was found to be at 485 nm and 570 nm and the linearity was found in concentration range of 10-50 μg/ml and 5-25 μg/ml respectively for method A and method B. The correlation coefficient was found to be 0.9986 and 0.9993 respectively for method A and method B. The methods were validated as per USP and ICH guidelines.
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