The manuscript describes validated Q-absorbance ratio method for the simultaneous estimation of brimonidine tartrate and timolol maleate in eye drop. Q-absorbance ratio method was based on the estimation of both the drugs at two wavelengths i.e. absorption maxima of one drug and iso-absorptive point. An absorption maximum of timolol maleate was 294.1 nm and iso-absorptive point was 270.0 nm in methanol. The linearity was obtained over the concentration range of 10–70 µg/ml for both the drug with mean recovery of 97.81±1.29% and 97.72±0.63% for brimonidine tartrate and timolol maleate, respectively. The method was found to be simple, sensitive, accurate, precise and economical and applicable for the simultaneous determination of brimonidine tartrate and timolol maleate in combined dosage form.
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